A gravity column is suitable for use with this resin. Product Z163961-1EA is an example of a gravity column. The glass column does not have a frit, so a glass-wool plug and sand are used. To plug the column, gently insert the glass-wool plug from the column top and push it down the column gently using a glass rod.
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form
solid
Quality Level
packaging
pkg of 500 g
technique(s)
HPLC: suitable
LPLC: suitable
ion chromatography: suitable
thin layer chromatography (TLC): suitable
surface area
~500 m2/g
matrix
styrene-divinylbenzene
matrix active group
polymer
particle size
250-850 μm
pore size
~1.30 mL/g pore volume
260 Å mean pore size
density
1.01 g/mL at 25 °C (true wet)(lit.)
separation technique
reversed phase
SMILES string
C(C)c3c(cccc3)C=C.c2(c(cccc2)C=C)C=C.c1(ccccc1)C=C
InChI
1S/C10H12.C10H10.C8H8/c2*1-3-9-7-5-6-8-10(9)4-2;1-2-8-6-4-3-5-7-8/h3,5-8H,1,4H2,2H3;3-8H,1-2H2;2-7H,1H2
InChI key
NWUYHJFMYQTDRP-UHFFFAOYSA-N
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General description
Diaion® HP-20 is a non-polar copolymer styrene-divynilbenzene adsorbent resin. It is a polymeric adsorbent used in reverse phase chromatography.[1] It has spherical particles with 0.5mm diameter.[2] Diaion HP-20 has been considered as the most effective adsorbent to eliminate toxic effects in fermentation.[3] It is a synthetic adsorbent used for preconcentration and separation of traces heavy metal ions.[4]
Application
Polyaromatic adsorbent resin for hydrophobic compounds: antibiotics, biomolecules; useful for desalting; broad application base.
Other Notes
Swelling in toluene = 30%
Legal Information
Diaion is a registered trademark of Mitsubishi Chemical Corp.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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What king of column can be used for this resin? Could you please explain how to plug the column glass tube? My guess is that a cotton plug and sand will not work well with backflow which is recommended for this resin.
1 answer-
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