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WGA3

Sigma-Aldrich

GenomePlex® WGA Reamplification Kit

Reamplification of WGA product with minimal bias

Synonym(s):

GenomePlex® whole genome amplification reamplification kit, Whole genome reamplification kit

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About This Item

UNSPSC Code:
41121800
NACRES:
NA.55

Quality Level

technique(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

General description

GenomePlex® WGA Reamplification Kit utilizes a proprietary amplification method that is based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. It allows the user to obtain even higher yields of desired DNA without disturbing the original, limited source of genomic DNA. The genomic DNA can be reamplified up to five cycles with minimal allele dropout producing an even higher yield. It provides the researcher to generate a representative, ~500-fold amplification of genomic DNA.

Application

GenomePlex® WGA Reamplification Kit has been used to generate DNA probes (μBm-probe and μBsm-probe) labeled with digoxigenin-11-dUTP. It has also been used in the reamplification of WGA2-amplified genomic DNA to generate >10 μg gDNA for microarray hybridization.

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

Kit Components Only

Product No.
Description

  • Deoxynucleotide Mix, 10 mM

Kit Components Also Available Separately

Product No.
Description
SDS

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .2 mLSDS

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mirella L Meyer-Ficca et al.
Chromosoma, 122(4), 319-335 (2013-06-05)
The mammalian sperm nucleus is characterized by unique properties that are important for fertilization. Sperm DNA retains only small numbers of histones in distinct positions, and the majority of the genome is protamine associated, which allows for extreme condensation and
Rita Scardino et al.
Genes, 11(4) (2020-04-05)
The history of each human chromosome can be studied through comparative cytogenetic approaches in mammals which permit the identification of human chromosomal homologies and rearrangements between species. Comparative banding, chromosome painting, Bacterial Artificial Chromosome (BAC) mapping and genome data permit
A multiplex PCR predictor. for aCGH success of FFPE samples.
Van Beers E.H., et al.
British Journal of Cancer, 94, 333-337 (2005)
Yun Xia et al.
Frontiers in genetics, 10, 1364-1364 (2020-02-11)
Chromosomal rearrangements have long fascinated evolutionary biologists for being widely implicated in causing genetic differentiation. Suppressed recombination has been demonstrated in various species with inversion; however, there is controversy over whether such recombination suppression would facilitate divergence in reciprocal translocation
Churamani Khanal et al.
Plant disease, 103(8), 1835-1842 (2019-06-14)
This study employed single nucleotide polymorphisms (SNPs) to determine the genetic variability present in 26 isolates of Rotylenchulus reniformis from Louisiana, Mississippi, Arkansas, South Carolina, Georgia, Hawaii, and Alabama. Genomic DNA from reniform nematode was extracted and increased quantitatively using

Articles

Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.

Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.

Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.

Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.

Protocols

GenomePlex® is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative amplification of genomic DNA

GenomePlex is a Whole Genome Amplification (WGA) method that allows the researcher to generate a representative, 500-fold amplification of genomic DNA

This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.

GenomePlex® Whole Genome Amplification has been used to amplify genomic DNA from soybean, corn, tomato, purple coneflower, and ginseng.

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