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SRP3242

Sigma-Aldrich

IL-2 from rat

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Aldesleukin, T-cell growth factor (TCGF)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

rat

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤0.4 ng/mL ED50

mol wt

15.3 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... IL2(116562)

General description

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. IL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues.

Biochem/physiol Actions

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues.

Sequence

MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAIAQSI ISTM

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 4.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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K A Smith
Science (New York, N.Y.), 240(4856), 1169-1176 (1988-05-27)
Interleukin-2 (IL-2), the first of a series of lymphocytotrophic hormones to be recognized and completely characterized, is pivotal for the generation and regulation of the immune response. A T lymphocyte product, IL-2 also stimulates T cells to undergo cell cycle
Xiumei Wen et al.
Molecular medicine reports, 24(5) (2021-09-10)
Acute pancreatitis (AP) is one of the most frequent gastrointestinal diseases and has no specific treatment. It has been shown that dysfunction of pancreatic acinar cells can lead to AP progression. Emodin is a natural product, which can alleviate the
Lindsey Chudley et al.
Cancer immunology, immunotherapy : CII, 63(11), 1199-1211 (2014-08-20)
Ex vivo ELISPOT and multimer staining are well-established tests for the assessment of antigen-specific T cells. Many laboratories are now using a period of in vitro stimulation (IVS) to enhance detection. Here, we report the findings of a multi-centre panel
Luiza M Araujo et al.
Arthritis & rheumatology (Hoboken, N.J.), 66(9), 2412-2422 (2014-06-10)
HLA-B27/human β2 -microglobulin (hβ2 m)-transgenic (B27-transgenic) rats develop an inflammatory disorder resembling spondyloarthritis, with accumulation of proinflammatory Th17 cells. Because Treg cells and Th17 cells have opposing effects in inflammatory disorders, we sought to determine whether biased expansion of Th17
Fang-Ting Lu et al.
Journal of autoimmunity, 61, 62-72 (2015-06-15)
Thymic CD4(+) FoxP3(+) regulatory T (Treg) cells are critical for the development of immunological tolerance and immune homeostasis and requires contributions of both thymic dendritic and epithelial cells. Although B cells have been reported to be present within the thymus

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