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SAB4200749

Sigma-Aldrich

Anti-Collagen Type III antibody, Mouse monoclonal

clone FH-7A, purified from hybridoma cell culture

Synonym(s):

Anti-COL3A1, Anti-Collagen alpha-1(III) chain

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

FH-7A, monoclonal

form

buffered aqueous solution

mol wt

~70 kDa

species reactivity

rat, human

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable
immunoblotting: suitable
immunofluorescence: suitable
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat skin sections.

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... COL3A1(1281)

General description

Anti-collagen type III antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the FH-7A hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c immunized mouse.
Monoclonal anti-collagen type III specifically recognizes native and denatured collagen type III from human 1 and rat 2 origin. It does not recognize collagen types I, II, IV, V, VI and X.
Collagen type III, also known as collagen α-1(III) (COL3A1), is encoded by the gene mapped to human chromosome 2q24.3-q31. It is the highly expressed collagen in blood vessels and hollow organs.

Immunogen

Human Collagen Type III

Biochem/physiol Actions

Collagen a-1(III) (COL3A1) serves as a potent adjunct marker for both differentiating fibroadenoma (FA) from phyllodes tumor (PT) and measuring malignant potential in PTs. Overexpression of the gene in glomerular leads to the development of collagen type III glomerulopathy. Mutations in the gene is associated with the development of familial aortic aneurysms and Ehlers-Danlos syndrome.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at -20°C. For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Transcriptome analysis of skin fibroblasts with dominant negative COL3A1 mutations provides molecular insights into the etiopathology of vascular Ehlers-Danlos syndrome.
Chiarelli N, et al.
PLoS ONE, 13 (2018)
A Mutation in the Gene for Type Ill Procollagen (COL3A1)
in a Family with Aortic Aneurysms
Kontusaari S, et al.
The Journal of Clinical Investigation, 86, 1465-1473 (1990)
Linkage mapping of the gene for type III collagen (COL3A1) to human chromosome 2q using a VNTR polymorphism.
Tiller GE, et al.
Genomics, 20, 275-277 (1994)
Glomerular Collagen V Codeposition and Hepatic Perisinusoidal Collagen III Accumulation in Canine Collagen Type III Glomerulopathy.
R?rtveit R, et al.
Veterinary Pathology, 52, 1134-1141 (2015)
Collagen type III a1 as a useful diagnostic immunohistochemical marker for fibroepithelial lesions of the breast.
Wang Y, et al.
Human Pathology, 57, 176-181 (2016)

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