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SAB4200724

Sigma-Aldrich

Anti-AGO2 antibody, Mouse monoclonal

clone AGO2-10, purified from hybridoma cell culture

Synonym(s):

Anti-Argonaute RISC catalytic component 2, Anti-Argonaute2, Anti-Eukaryotic translation initiation factor 2C 2, Anti-PAZ Piwi domain protein (PPD), Anti-Protein Argonaute 2, Anti-eIF2C 2

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AGO2-10, monoclonal

form

buffered aqueous solution

mol wt

~95 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using extract of HEK-293T cells transfected with human AGO2
immunoprecipitation (IP): 10-20 μg/test using HeLa cells extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AGO2(27161)

General description

Anti-AGO2 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the AGO2-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with synthetic peptide from the C-terminal region of human AGO2 protein, conjugated to keyhole limpet hemocyanin (KLH). AGO2 is also known as protein argonaute 2, argonaute RNA-induced silencing complex (RISC) catalytic component 2, eukaryotic translation initiation factor 2C 2 (eIF2C2) and PAZ Piwi domain protein (PPD). Argonaute 2 (AGO2) belongs to the Argonaute family. It is located on human chromosome 8q24. Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies.

Immunogen

synthetic peptide from the C-terminal region of human AGO2 protein, conjugated to KLH

Application

Anti-AGO2 antibody, Mouse monoclonal has been used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Argonaute 2 (AGO2) exhibits intrinsic endonuclease activity. It can directly cleave mRNAs in a small RNA-guided manner. AGO2 plays a central role in RNA silencing processes as essential catalytic components of the RNA-induced silencing complex (RISC). It has an important role in stabilization of circulating microRNAs (miRNAs) in exosomes from extracellular body fluids.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The RNA silencing endonuclease Argonaute 2 mediates specific antiviral immunity in Drosophila melanogaster
Van RRP, et al.
Genes & Development, 20(21), 2985-2995 (2006)
Argonaute2 promotes tumor metastasis by way of up-regulating focal adhesion kinase expression in hepatocellular carcinoma
Cheng N, et al.
Hepatology, 57(5), 1906-1918 (2013)
Argonaute2 expression is post-transcriptionally coupled to microRNA abundance
Martinez NJ and Gregory RI
RNA, 19(5), 605-612 (2013)
Zhenyu Li et al.
Cancer cell international, 20, 294-294 (2020-07-17)
Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored.
Mahmoud Kandeel et al.
PloS one, 9(5), e94538-e94538 (2014-05-03)
Gene silencing and RNA interference are major cellular processes that control gene expression via the cleavage of target mRNA. Eukaryotic translation initiation factor 2C2 (EIF2C2, Argonaute protein 2, Ago2) is considered to be the major player of RNAi as it

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