Skip to Content
Merck
All Photos(2)

Key Documents

SAB1300127

Sigma-Aldrich

Anti-PACSIN2 (C-term) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-Protein kinase C and casein kinase substrate in neurons protein 2

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

mouse, human

technique(s)

immunohistochemistry: 1:50-1:100
indirect ELISA: 1:1000
indirect immunofluorescence: 1:10-1:50
western blot: 1:100-1:500

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PACSIN2(11252)

General description

Protein kinase C and casein kinase substrate in neurons 2 (PACSIN2), also termed syndapin II exhibits a vesicle-like cytoplasmic distribution and is ubiquitously expressed. PACSIN is phosphorylated by casein kinase 2 (CK2) and protein kinase C (PKC). The protein contains EFC/F-BAR (Fes/CIP4 homology - Bin/amphiphysin/Rvs ) domain (1 FCH domain) and 1 SH3 (Src homology 3) domain. In human chromosome, the gene PACSIN2 is localized on 22q13.2.

Immunogen

PACSIN2 (NP_009160, 347-381)
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PACSIN2.

Application

Anti-PACSIN2 (C-term) antibody produced in rabbit has been used in immunostaining.

Biochem/physiol Actions

Protein kinase C and casein kinase substrate in neurons 2 (PACSIN2) may play a role in vesicle formation and transport and organization of cytoskeleton. This protein homo- and hetero-aggregates with other PACSINs and thus acts as membrane associated adapter protein. It also binds dynamin 1, synaptojanin, synapsin 1 and the neural Wiskott-Aldrich syndrome protein (N-WASP). Pacsin2 is crucial for the formation and maintenance of kidney associated tubules. Pacsin2 is involved with caveolae biogenesis in plasma membrane. Pacsin2 regulates cell migration and spreading by binding with Rac1 (Ras-related C3 botulinum toxin substrate 1) protein. Pacsin2 is involved in microspike formation and tubulation like filopodia, lamellopodia in the cell.

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Pacsin 2 is recruited to caveolae and functions in caveolar biogenesis
Hansen CG, et al.
Journal of Cell Science, 124(16), 2777-2785 (2011)
Mapping of the basic amino-acid residues responsible for tubulation and cellular protrusion by the EFC/F-BAR domain of pacsin2/Syndapin II
Shimada A, et al.
Febs Letters, 584(6), 1111-1118 (2010)
Novel activating BRAF fusion identifies a recurrent alternative mechanism for ERK activation in pediatric Langerhans cell histiocytosis
Zarnegar S, et al.
Pediatric Blood & Cancer, 65(1), e26699-e26699 (2018)
Polycystin-1 regulates actin cytoskeleton organization and directional cell migration through a novel PC1-Pacsin 2-N-Wasp complex
Yao G, et al.
Human Molecular Genetics, 23(10), 2769-2779 (2014)
PACSIN 2, a novel member of the PACSIN family of cytoplasmic adapter proteins
Ritter B, et al.
Febs Letters, 454(3), 356-362 (1999)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service