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R4654

Sigma-Aldrich

Anti-Rab5 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Ras-Related Protein Rab5A

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~24 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH-3T3 cells.
western blot: 2.5-5 μg/mL using whole extract of human A431 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... RAB5A(5868)
mouse ... Rab5a(271457)
rat ... Rab5a(64633)

General description

Ras related protein consists of three isoforms such as Rab5a, Rab5b and Rab5c. Rab5a is encoded by the gene mapped to human chromosome 3p24.3. This gene codes for 23.66 kDa Rab5a protein.

Immunogen

synthetic peptide corresponding to amino acid residues 176-188 of human Rab5 with C-terminal added cysteine, conjugated to KLH.

Application

Anti-Rab5 antibody produced in rabbit has been used in western blotting and immunocytochemistry.

Biochem/physiol Actions

Rab proteins are members of small GTPases control the exocytosis and endocytosis and regulate vesicular transport. Rab5 regulates endocytic pathway in mammalian cells and an activator of autophagy. It mediates macroautophagy of cells expressing aggregates of mutant Huntingtin protein.
Rab5a is essential for epidermal growth factor (EGF)-mediated EGF receptor degradation. Overexpression of Rab5a is associated with the development of variety of cancers such as breast cancer, lung cancer, hepatocellular carcinoma (HCC) and ovarian cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Site-specific monoubiquitination downregulates Rab5 by disrupting effector binding and guanine nucleotide conversion
Shin D, et al.
eLife, 6, e29154-e29154 (2017)
Rab5a promotes the migration and invasion of hepatocellular carcinoma by up-regulating Cdc42
Yang X, et al.
International Journal of Clinical and Experimental Pathology, 11(1), 224-231 (2018)
J Somsel Rodman et al.
Journal of cell science, 113 Pt 2, 183-192 (2000-01-14)
Endocytosis is characterized by vesicular transport along numerous pathways. Common steps in each pathway include membrane budding to form vesicles, transport to a particular destination, and ultimately docking and fusion with the target membrane. Specificity of vesicle targeting is rendered
Lysosomal network proteins as potential novel CSF biomarkers for Alzheimer?s disease
Armstrong A, et al.
Neuromolecular Medicine, 16(1), 150-160 (2014)
Valentina Di Felice et al.
Cells, 11(9) (2022-05-15)
Currently, no commercially available drugs have the ability to reverse cachexia or counteract muscle wasting and the loss of lean mass. Here, we report the methodology used to develop Physiactisome-a conditioned medium released by heat shock protein 60 (Hsp60)-overexpressing C2C12

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