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MAK136

Sigma-Aldrich

Monoamine Oxidase Activity Assay Kit

Monoamine Oxidase Activity Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

MAO Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

neurological disorders

storage temp.

−20°C

Gene Information

human ... MAOA(4128) , MAOB(4129)
mouse ... MAOA(17161) , MAOB(109731)
rat ... MAOA(29253) , MAOB(25750)

Related Categories

General description

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. In mammals, MAO consists of two isoenzymes, MAO-A and MAO-B. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAO in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drug prescribed for the treatment of depression.

Application

Monoamine Oxidase Activity Assay Kit has been used to estimate MAO-A (monoamine oxidase A)[1][2], MAO-B (monoamine oxidase B)[1] and ALDH (aldehyde dehydrogenase) activities.[2]

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the determination of MAO activity in biological samples and for the screening of MAO inhibitors. Can determine either MAO-A, MAO-B or both MAO-A and MAO-B activity.

Principle

The Monoamine Oxidase Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity in biological samples. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (λex = 530/λem = 585 nm). The assay is simple, sensitive, stable, and high-throughput adaptable. Unit definition: one unit of MAO catalyzes the formation of 1 μmole of H2O2 per minute under the assay conditions.

Storage Class Code

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
136CE01A02
136CD07A06
136CE02A08
136CD05A15
136CC07A25

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Lovedeep Singh et al.
Metabolic brain disease, 36(1), 111-121 (2020-09-02)
Pain and depression are often co-existing pathological states that promote mutual severity resulting in limited efficacy of current treatment strategies. Thus, there is a need to develop an efficacious alternate treatment regimen for pain-depression dyad. Skimmetin and osthole are molecules
Proteome Profiling of Primary Pancreatic Ductal Adenocarcinomas Undergoing Additive Chemoradiation Link ALDH1A1 to Early Local Recurrence and Chemoradiation Resistance.
Oria V O, et al.
Translational Oncology, 11(6), 1307-1322 (2018)
Umut Salgin-Goksen et al.
Journal of medicinal chemistry, 64(4), 1989-2009 (2021-02-04)
Thirty compounds having 1-[2-(5-substituted-2-benzoxazolinone-3-yl) acetyl]-3,5-disubstitutedphenyl-2-pyrazoline structure and nine compounds having N'-(1,3-disubstitutedphenylallylidene)-2-(5-substituted-2-benzoxazolinone-3-yl)acetohydrazide skeleton were synthesized and evaluated as monoamine oxidase (MAO) inhibitors. All of the compounds exhibited selective MAO-A inhibitor activity in the nanomolar or low micromolar range. The results of
Juho-Matti Renko et al.
Molecular neurobiology, 55(8), 6755-6768 (2018-01-20)
Neurotrophic factors (NTFs) hold potential as disease-modifying therapies for neurodegenerative disorders like Parkinson's disease. Glial cell line-derived neurotrophic factor (GDNF), cerebral dopamine neurotrophic factor (CDNF), and mesencephalic astrocyte-derived neurotrophic factor (MANF) have shown neuroprotective and restorative effects on nigral dopaminergic
Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF) Elevates Stimulus-Evoked Release of Dopamine in Freely-Moving Rats.
Renko J M, et al.
Molecular Neurobiology, 1-14 (2018)
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Questions

1–5 of 5 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. What is the concentration of the Dye Reagent stock that is included in the kit?

    1 answer

    1. The Dye Reagent that is provided as a component of this kit is a solution in Dimethyl Sulfoxide (DMSO). This product is made by a third party; the original manufacturer holds information on the identity and concentration of the dye, as well as similar information on other components of the kit, as proprietary.

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  4. What is the concentration of the p-tyramine stock and the HRP enzyme that is included in the kit?

    1 answer

    1. The concentration of the p-tyramine included in the kit is 100mM, and the enzyme concentration is 0.02 U/uL.

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  5. Is it possible to determine if this kit would be suitable for the quantitation of MAO-N?

    1 answer

    1. This kit is not tested with MAO-N. It is important to note that this kit was specifically developed with MAO-A and MAO-B in mind, and it is uncertain whether other enzymes, such as MAO-N, would be compatible with the substrate.

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