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I2658

Sigma-Aldrich

Anti-phospho-Insulin Receptor Substrate-1 (pTyr612) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-phospho-IRS-1 (pTyr612)

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human

packaging

vial of 100 μL

technique(s)

western blot: suitable

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

phosphorylation (pTyr612)

Gene Information

human ... IRS1(3667)

General description

Assay Dependent. It is recommended that the user determine the appropriate working dilution for their process.
Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. . Phosphorylation of Tyr612 and Tyr632 fully activate the PI3K signalling pathway in response to insulin and induce translocation of GLUT4. Thus IRS-1 protein plays a vital role in mediating the metabolic actions of insulin via the PI3K pathway. Additionally, IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. The transcriptional activity of IRS-1 is involved in the development of breast and prostate cancers
The antibody recognizes human IRS-1 (pTyr612). It may cross react with in mouse, rat and chicken (100% homologous) IRS-1 (pTyr612).

Immunogen

synthetic phosphopeptide derived from the region of insulin receptor substrate-1 (IRS-1) phosphorylated at tyrosine 612.

Application

Anti-phospho-IRS-1 (pTyr612) antibody may be used for detection by immunoblotting.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in Dulbecco′s PBS, pH 7.3, 50% glycerol containing 1 mg/ml BSA and ≤ 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Vanessa R Haynes et al.
Journal of lipid research, 60(8), 1350-1364 (2019-06-17)
Defects in the gene coding for dysferlin, a membrane-associated protein, affect many tissues, including skeletal muscles, with a resultant myopathy called dysferlinopathy. Dysferlinopathy manifests postgrowth with a progressive loss of skeletal muscle function, early intramyocellular lipid accumulation, and a striking
Susanne Lager et al.
Physiological reports, 2(2), e00242-e00242 (2014-04-20)
As in humans, obesity during pregnancy in mice results in elevated maternal insulin levels and metabolic programming of offspring. mTOR signaling regulates amino acid transport and may function as a placental nutrient sensor. Because obesity is a condition with increased
D L Esposito et al.
Endocrinology, 142(7), 2833-2840 (2001-06-21)
To examine contributions of specific YXXM motifs in human insulin receptor substrate-1 (IRS-1) to mediating the metabolic actions of insulin, we studied IRS-1 mutants containing various substitutions of Phe for Tyr. In transfected NIH-3T3(IR) cells, insulin stimulation caused a 5-fold
Byungyong Ahn et al.
The Journal of clinical investigation, 126(9), 3567-3579 (2016-08-09)
Intramuscular lipid accumulation is a common manifestation of chronic caloric excess and obesity that is strongly associated with insulin resistance. The mechanistic links between lipid accumulation in myocytes and insulin resistance are not completely understood. In this work, we used
Xiaojun Zhou et al.
British journal of pharmacology, 176(3), 478-490 (2018-11-25)
Sulfonylureas (SUs) have been suggested to have an insulin-independent blood glucose-decreasing activity due to an extrapancreatic effect. However, a lack of adequate in vivo evidence makes this statement controversial. Here, we aimed to evaluate whether glimepiride has extrapancreatic blood glucose-lowering

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