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GERPN3004

ECL Detection Reagents

Cytiva RPN3004, pack of 1 ea

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About This Item

UNSPSC Code:
41105300
NACRES:
NA.31

packaging

pack of 1 ea

manufacturer/tradename

Cytiva RPN3004

storage temp.

2-8°C

Related Categories

General description

The world’s first commercially available chemiluminescent detection reagent for Western blotting. Amersham ECL detection reagent uses enhanced luminol-based detection suitable for all routine confirmatory Western blotting experiments.

Based on the enhanced chemiluminescent reaction of luminol with horseradish peroxidase, ECL substrate can be used to detect probes which have been labelled either directly or indirectly with horseradish peroxidase. Rapid light output enables results to be achieved in 10 to 15 minutes. It is the substrate of choice for target amounts above 500 fg.

High sensitivity and fast results:

  • Detects up to 10 to 12 ng of protein, which is ~10× more than colorimetric methods
  • Optimized to use with Amersham Protran nitrocellulose (NC) membranes
  • Delivers results in just a few minutes, then image using a CCD imager like the Amersham ImageQuant 800 or Amersham Hyperfilm ECL

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

ECL is a trademark of Cytiva

Storage Class Code

12 - Non Combustible Liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service