EMT6/VCR/R
96042323, mouse unknown/unspecified, Epithelial-like
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
41106514
Recommended Products
product name
EMT6/VCR/R, 96042323
biological source
mouse unknown/unspecified
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse mammary, drug-resistant
Cell Line Description
EMT6/VCR/R is a drug-resistant variant of the mouse mammary tumour cell line EMT6/P (Sigma Catalogue number. 96042344). It was derived from the parent line by continuous growth in increasing concentrations of vincristine. EMT6/VCR/R was reported to be cross-resistant to other drugs of the multidrug-resistance (MDR) phenotype, showed reduced intracellular drug accumulation and hyperexpression of P-glycoprotein.
Application
Drug-resistance studies
Culture Medium
MEM(EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1μg/ml vincristine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37ºC. It is recommended to resusitate cells in drug-free medium. Add drug after growth is established.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service