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product name
ARH 77, 88121201
biological source
human blood
growth mode
Suspension
karyotype
Hypodiploid
morphology
Lymphoblast
products
Immunoglobulin G (IgG1) (kappa)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human plasma cell leukaemia
Cell Line Description
Derived from a 33 year old female with plasma cell leukaemia. The cells synthesise IgG1 (kappa-light chain) and are EBNA positive.Although the cells are reported to originate from a patient with a plasma cell leukaemia , it has now been shown that this is in fact an EBV transformed B lymphoblastoid cell line .
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8,9.3
TPOX: 8
vWA: 17
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8,9.3
TPOX: 8
vWA: 17
Culture Medium
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Maintain cultures between 1-4x100,000 cells/ml; 5% CO2; 37°C. Maximum cell density 1-2x1,000,000 cells/ml.
Other Notes
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Certificates of Analysis (COA)
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