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B1645

Sigma-Aldrich

Anti-Bovine IgG (whole molecule) antibody produced in goat

whole antiserum

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

secondary antibodies

clone

polyclonal

contains

15 mM sodium azide

technique(s)

quantitative precipitin assay: 2.8 mg/mL

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids.

Specificity

The antiserum shows a single arc of precipitation versus bovine serum and bovine IgG by immunoelectrophoresis (IEP). This antiserum has not been assayed for interspecies cross reactivity.

Application

Anti-Bovine IgG (whole molecule) antibody produced in goat has been used in:
  • quantitative precipitation assays and in various immunosensor methods to detect bovine IgG in serum and milk
  • immuno-reaction assay
  • indirect sandwich enzyme linked immunosorbent assay (ELISA)
It has also been used in immuno-reaction assay.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

Physical form

Supplied as a liquid with 0.1% sodium azide (see MSDS) as a preservative.

Preparation Note

treated to remove lipoproteins

Storage and Stability

For continuous use, store at 0-5 °C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Use of sandwich IgG ELISA for the detection and quantification of adulteration of milk and soft cheese
<BIG>Hurley LP, et al.</BIG>
International dairy journal, 7(16), 805-812 (2006)
Mauro Tomassetti et al.
Journal of pharmaceutical and biomedical analysis, 73, 90-98 (2012-05-09)
Within the framework of research carried out by our team aimed at developing new immunological methods to determine proteins such as immunoglobulins G in different biological matrixes, for instance, serum and milk, tests were performed on several immunosensors based on
Bismuth film electrode as sensing platform for IgE-anti-IgE interactions
Anik U, et al.
Electroanalysis, 23(10), 2379-2385 (2011)
Mahmoud K Eldahshoury et al.
Journal of dairy science, 106(9), 5908-5915 (2023-07-22)
The demand for commercially available human breast milk has significantly increased in recent years. For various reasons, a significant amount of commercially available human breast milk is being adulterated with other types of milk. This fraudulent practice poses a threat
IL-21-induced isotype switching to IgG and IgA by human naive B cells is differentially regulated by IL-4
Avery DT, et al.
Journal of Immunology, 181(3), 1767-1779 (2008)

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