73737

4-Nitrophenyl phosphate bis(tris) salt

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

• Synonym(s): p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt
• Linear Formula: (C₆ H₄ N O₆ P)( C₄ H₁₂ N O₃)₂
• CAS Number: 68189-42-4
• Molecular Weight: 461.36
• Beilstein: 1578142
• EC Number: 269-198-6
• MDL number: MFCD00037009
• UNSPSC Code: 12352204
• PubChem Substance ID: 57652488
• NACRES: NA.32

Properties

• product name: 4-Nitrophenyl phosphate bis(tris) salt, for the determination of phosphatase, ≥97.0% (enzymatic)
• Assay: ≥97.0% (enzymatic)
• form: powder or crystals
• quality: for the determination of phosphatase
• impurities: ≤0.1% free nitrophenol; ≤6% water
• solubility: H₂O: 0.1 g/mL, clear
• storage temp.: 2-8°C
• SMILES string: NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O
• InChI: 1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2
• InChI key: XXAXKCWOTRABOW-UHFFFAOYSA-N

Description

Application

4-Nitrophenyl phosphate bis(tris) salt has been used to determine the enzyme activity of acid and alkaline phosphatase in cell lysates.

Biochem/physiol Actions

4-Nitrophenyl phosphate is a non-proteinaceous and non-specific chromogenic substrate, preferred for alkaline phosphatase (ALP) in enzyme-linked immunosorbent assay (ELISA). It is widely used to assay protein tyrosine phosphatases (PTPs). Upon cleavage, the substrate produces a yellow product that is spectrophotometrically measured.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves