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PHR1789

Supelco

Doxorubicin Hydrochloride

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym(s):

Doxorubicin hydrochloride, DOX, Hydroxydaunorubicin hydrochloride

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About This Item

Empirical Formula (Hill Notation):
C27H29NO11 · HCl
CAS Number:
25316-40-9
Molecular Weight:
579.98
Beilstein:
4229251
EC Number:
246-818-3
MDL number:
MFCD00077757
UNSPSC Code:
41116107
PubChem Substance ID:
329823614
NACRES:
NA.24
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grade

certified reference material
pharmaceutical secondary standard

Quality Level

300

Agency

traceable to BP 990
traceable to Ph. Eur. D2975000
traceable to USP 1225703

API family

doxorubicin

form

powder

CofA

current certificate can be downloaded

packaging

pkg of 200 mg

technique(s)

HPLC: suitable
gas chromatography (GC): suitable

mp

216 °C (dec.) (lit.)

application(s)

pharmaceutical (small molecule)

storage temp.

-10 to -25°C

SMILES string

Cl[H].COc1cccc2C(=O)c3c(O)c4C[C@](O)(C[C@H](O[C@H]5C[C@H](N)[C@H](O)[C@H](C)O5)c4c(O)c3C(=O)c12)C(=O)CO

InChI

1S/C27H29NO11.ClH/c1-10-22(31)13(28)6-17(38-10)39-15-8-27(36,16(30)9-29)7-12-19(15)26(35)21-20(24(12)33)23(32)11-4-3-5-14(37-2)18(11)25(21)34;/h3-5,10,13,15,17,22,29,31,33,35-36H,6-9,28H2,1-2H3;1H/t10-,13-,15-,17-,22+,27-;/m0./s1

InChI key

MWWSFMDVAYGXBV-RUELKSSGSA-N

Gene Information

human ... TOP2A(7153)

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General description

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Doxorubicin Hydrochloride is an anthracycline antibiotic, known for its broad range anti-cancer activities.

Application

This pharmaceutical secondary standard can also be used as follows:
  • Development of a reversed-phase high-performance liquid chromatographic (RP-HPLC) method combined with UV detection to determine doxorubicin hydrochloride in plasma samples[1]
  • Electrochemical determination of doxorubicin by spinel-structured cobalt ferrite (CoFe2O4) modified carbon paste electrode in biological fluid and injection samples[2]
  • Determination of doxorubicin hydrochloride in human plasma samples using a graphene quantum dots (GQDs) based electrochemical sensor
  • RP-HPLC method-based quantitative analysis of doxorubicin hydrochloride in vacuum foam dried formulations[3]
  • Voltammetric determination of doxorubicin hydrochloride and dastinib using zinc oxide (ZnO) nanoparticle/1-butyl-3-methylimidazolium tetra-fluoroborate modified carbon paste electrode (ZnO-NPs/BMTFB/CPE) in their combined injection samples[4]

Analysis Note

These secondary standards offer multi-traceability to the USP, EP and BP primary standards, where they are available.

Footnote

To see an example of a Certificate of Analysis for this material enter LRAB3692 in the Documents slot below. This is an example certificate only and may not be the lot that you receive.

Recommended products

Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H340,H350,H360FD

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Muta. 1B - Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

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Development and validation of RP-HPLC method for determination of doxorubicin hydrochloride from vacuum foam dried formulation
Hajare AA, et al.
Research Journal of Pharmacy and Technology, 9, 1352-1356 (2016)
A simple HPLC bioanalytical method for the determination of doxorubicin hydrochloride in rat plasma: application to pharmacokinetic studies
Dharmalingam SR, et al.
Tropical Journal of Pharmaceutical Research, 13, 409-415 (2014)
Fast and sensitive determination of doxorubicin using multi-walled carbon nanotubes as a sensor and CoFe2O4 magnetic nanoparticles as a mediator
Taei M, et al.
Microchimica Acta, 183, 49-56 (2016)
Simultaneous determination of doxorubicin and dasatinib as two breast anticancer drugs uses an amplified sensor with ionic liquid and ZnO nanoparticle
Alavi-Tabari SAR, et al.
Journal of Electroanalytical Chemistry, 811, 84-88 (2018)
Yi-Fang Yang et al.
The Kaohsiung journal of medical sciences, 35(7), 408-416 (2019-04-20)
Breast cancer is the most common female-specific malignancy in Taiwan and developed countries worldwide, and its incidence continues to grow. 14-3-3ε (YWHAE), which belong to 14-3-3 family, it has been reported up-regulated in breast cancer tissues. However, the clinical implication
View All Related Papers

Questions

  1. How should the doxorubicin be dissolved? I have seen DMSO, but what is the optimal solution to dilute the DMSO and at what percentage?

    1 answer

    1. This product is not tested for solubility. However, information available for other Doxorubicin HCl products will apply.
      D1515, Doxorubicin HCl, is soluble in water and pure DMSO at 50 mg/ml.
      Solutions are sensitive to light and will degrade over long-term exposure.
      https://www.sigmaaldrich.com/product/sigma/d1515

      D5225, Doxorubicin, Ready-Made solution is a 2mg/ml solution in pure DMSO.
      The product is stored at 20°C and is assigned a retest schedule of every 2 years.
      https://www.sigmaaldrich.com/product/sigma/d5220

      Solutions appear to be reasonably stable when protected from light.
      See the link below to review a publication regarding the solution stability of Doxorubicin HCl.:
      https://pubmed.ncbi.nlm.nih.gov/517541/

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