90210

D-(+)-Trehalose dihydrate

≥99.0%, suitable for microbiology, composed of two α-glucose units that is used as a protectant, stabilizer and to support proper folding

• Synonym(s): Trehalose dihydrate, a-d-glucopyranosyl-a-d-glucopyranoside, α,α-Trehalose, α-D-Glucopyranosyl-α-D-glucopyranoside
• Empirical Formula (Hill Notation): C₁₂H₂₂O₁₁ · 2H₂O
• CAS Number: 6138-23-4
• Molecular Weight: 378.33
• Beilstein: 5322018
• EC Number: 202-739-6
• MDL number: MFCD00071594
• UNSPSC Code: 41106212
• PubChem Substance ID: 329769859
• NACRES: NA.85

Properties

• Quality Level: 200
• Assay: ≥99.0% (HPLC); ≥99.0%
• form: powder
• optical activity: [α]20/D +176 to +186°, c = 2% in H₂O
• mol wt: 378.33 g/mol
• impurities: ≤0.1% glucose
• ign. residue: ≤0.1% (as SO₄)
• solubility: H₂O: 0.1 g/mL, clear, colorless
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: As: ≤0.1 mg/kg; Ca: ≤200 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Mg: ≤10 mg/kg; Mn: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Zn: ≤10 mg/kg
• application(s): microbiology
• SMILES string: [H]O[H].[H]O[H].OC[C@H]1O[C@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12;;/h3-20H,1-2H2;2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-;;/m1../s1
• InChI key: DPVHGFAJLZWDOC-PVXXTIHASA-N

Description

General description

D-(+)-Trehalose is a disaccharide composed of two α-glucose units. It is used as a cryoprotectant in a variety of cell freezing media. Used as a protectant in transportation media. It predominantly exists among the species that support anhydrobiosis (life without water). It serves as a carbohydrate reserve in microorganisms and protects them from adverse conditions, such as heat, cold, dehydration, desiccation, and oxidation. Trehalose acts as a chemical chaperone affecting protein folding. It is known to prevent the generation of amyloids and accumulation of β-amyloid in vitro. A similar effect of trehalose has been observed with Huntingtin protein aggregation. Thus, it might serve as a protective agent in Alzheimer′s disease and Huntington′s disease. Trehalose stimulates the better initial growth of Salmonella cells.

Application

D-(+)-Trehalose can be used as a carbohydrate reserve in certain cell cultures to tide over environmental stresses. It can also be used in biopharmaceutical monoclonal antibody formulations and as a protein stabilizer.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves