60634

Catalase from Micrococcus lysodeikticus

solution, dark brown

• CAS Number: 9001-05-2
• MDL number: MFCD00081483
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: bacterial (Micrococcus lysodeikticus)
• Quality Level: 200
• form: solution
• specific activity: 65,000-150,000 U/mL
• mol wt: M_r ~230000
• technique(s): microbe id | metabolite detection: suitable
• color: dark brown
• solubility: 0.05 M phosphate buffer pH 7.0: soluble, clear, brown (1:10)
• Protein ID accession no.: UTX35086
• UniProt accession no.: P29422
• shipped in: wet ice
• storage temp.: 2-8°C
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• InChI key: NUVBSKCKDOMJSU-UHFFFAOYSA-N

Description

General description

Research area: Cell Signaling

Catalase (CAT) is an antioxidant enzyme, encoded by the CAT gene family. The enzyme is ubiquitously expressed in most of the aerobic organisms.

Application

Catalase from Micrococcus lysodeikticus has been used to eliminate excess of hydrogen peroxide production during sugar analog synthesis by in vitro biocatalytic cascade. It has also been used as a standard to evaluate oxidative stress by indirectly measuring catalytic activity.

Biochem/physiol Actions

Catalase rapidly converts hydrogen peroxide to oxygen and water. It exhibits antioxidant activity and elicits a defense mechanism against reactive oxygen species (ROS). Catalase plays a role in neutralizing noxious hydrogen peroxide in nature as well as in various industries such as textile, pharma, and dairy. Micrococcus lysodeikticus catalase is a heme-containing protein.Catalase, along with various key cellular systems involved in hydrogen peroxide (H₂O₂) processing, plays a key role in regulating cellular redox balance and aids in cellular and intercellular signaling.

Unit Definition

1 U corresponds to the amount of enzyme which decomposes 1 μmol H₂O₂ per minute at pH 7.0 and 25 °C

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves