10102105001

Adenosine Deaminase (ADA)

from calf intestine

• Synonym(s): ADA
• Enzyme Commission number: 3.5.4.4 (BRENDA, IUBMB)
• UNSPSC Code: 12352204

Properties

• biological source: bovine (calf) intestine
• Quality Level: 100
• form: solution
• specific activity: ~200 units/mg protein (At 25 °C with adenosine as the substrate.)
• mol wt: 230-440 kDa (high); 30-47 kDa (low )
• packaging: pkg of 2 mL (10 mg)
• manufacturer/tradename: Roche
• optimum pH: 7.0-7.4
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Adenosine Deaminase (ADA) is present majorly in the proximal small intestine and the duodenum.[1] It is also called Adenosine aminohydrolase.[2] It is a zinc metalloenzyme and exists as a high (230−440 kDa) and low molecular weight (30−47 kDa) isoforms.[3]

Adenosine deaminase (ADA) is a zinc metalloenzyme.[4]

Application

Adenosine Deaminase (ADA) has been used as a component of macrophage- serum-free media (SFM) to treat macrophages.[5][6]

Adenosine deaminase has been used to pre-treat primary Kupffer cells.[7]

Biochem/physiol Actions

Adenosine Deaminase (ADA) catalyzes the deamination of adenosine analogs to the corresponding inosine analogs.[2] It is present in high levels in colorectal cancer and is a potential marker in tuberculous pleuriti.[2] ADA deficiency affects T-and B-lymphocytes resulting in severe combined immunodeficiency (SCID) leading to lymphocytopenia.[8]

Adenosine deaminase (ADA) is involved in the deamination of adenosine to inosine and 2′ deoxyadenosine to 2′-deoxyinosine.[9]

Quality

Contaminants: <0.01% alkaline phosphatase, AMP-deaminase, guanase, and nucleoside phosphorylase each

Physical form

Solution in 50% glycerol (v/v), 10 mM potassium phosphate, pH approximately 6

Preparation Note

Storage conditions (working solution): ADA (all preparations) may be dialyzed, without loss of activity, at 2 to 8 °C against 20 mM phosphate buffer, pH 7. The dialyzed preparation is stable at -15 to -25 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): does not flash
• Flash Point(C): does not flash