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MABT889

Sigma-Aldrich

Anti-CTHRC1 Antibody, clone Vli-55

clone Vli-55, from rabbit

Synonym(s):

Collagen triple helix repeat-containing protein 1, Protein NMTC1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

Vli-55, monoclonal

species reactivity

rat, mouse, human, porcine

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

mouse ... Cthrc1(68588)

General description

Collagen triple helix repeat-containing protein 1 (UniProt Q96CG8; also known as Protein NMTC1) is encoded by the CTHRC1 gene (ORF UNQ762/PRO1550; Gene ID 115908) in human. CTHRC1 is a secreted glycoprotein with 12 repeats of the Gly-X-Y motif (a.a. 58-93 based on the pro-form seqeunce) that is reported to increase cell motility and promote cell migration by limiting the deposition of collagen matrix. CTHRC1 is normally not expressed in blood vessels, but is induced in adventitial cells in remodeling arteries, as well as in dermal fibroblasts during wound healing. Upregulatetd CTHRC1 level is also observed in many human solid tumors, including non-small-cell lung cancer (NSCLC), gastric cancer, hepatocellular cancer, breast cancer, pancreatic cancer, and colorectal cancer (CRC). CTHRC1 is initially produced with a signal peptide sequence (a.a. 1-30), the removal of which yields the 213-amino acid (a.a. 31-243) mature protein.

Specificity

Clone Vli-55 stained the midbrain region of wild-type, but not Cthrc1-null mice (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142). Expected to react with all three spliced isoforms of human CTHRC1 reported by UniProt (Q96CG8).

Immunogen

Epitope: Near C-terminus.
Linear peptide corresponding to a C-terminal sequence of human CTHRC1.

Application

Anti-CTHRC1 Antibody, clone Vli-55 is an antibody against CTHRC1 for use in Western Blotting, Immunohistochemistry.
Immunohistochemistry Analysis: Clone Vli-55 hybridoma culture supernatant (1:250 dilution) immunostained the paraventricular nucleus of the hypothalamus in mouse brain tissue sections (Courtesy of Dr. Volkhard Lindner, Tufts University School of Medicine, ME, USA).
Western Blotting Analysis: Representative lots detected exogenously expressed human and rat CTHRC1 in lysates from transfected cells (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449; Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Western Blotting Analysis: A representative lot detected the overexpressed Cthrc1 in plasma samples from Cthrc1 trangenic mice, as well as immunoprecipitated CTHRC1 from normal human plasma samples (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot detected increased Cthrc1 expression in interstitial cells and activated fibroblasts of various remodeling tissues from wild-type, but not Cthrc1-null mice following angiotensin II infusion by immunohistochemistry staining of paraformaldehyde-fixed, paraffin-embedded sections (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449).
Immunohistochemistry Analysis: Representative lots immunostained Cthrc1-positive cells in paraformaldehyde-fixed, paraffin-embedded human, pig, and rat pituitary gland tissue sections (Duarte, C.W., et al. (2014). PLoS One.;9(6):e100449; Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot immunostained adventitial cells of remodeling renal artery, dermal cells in skin wounds, embryonic cartilage, as well as midbrain of wild-type, but not Cthrc1-null mice, using paraformaldehyde-fixed, paraffin-embedded tissue sections (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Immunohistochemistry Analysis: A representative lot localized Cthrc1 immunoreactivity in various regions of paraformaldehyde-fixed, paraffin-embedded mouse and pig brain sections (Stohn, J.P., et al. (2012). PLoS One. 7(10):e47142).
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 10 µg/mL of this antibody detected CTHRC1 in 10 µg of human brain tissue lysate.

Target description

~25 kDa observed. 23.08 kDa (isoform 1), 25.16 kDa (isoform 2), 24.77 kDa (isoform 3) calculated.

Physical form

Format: Purified
Protein G Purified
Purified rabbit monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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