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MABC293

Sigma-Aldrich

Anti-NLRP10 Antibody, clone 8H2

clone 8H2, from rat

Synonym(s):

NACHT, LRR and PYD domains-containing protein 10, Nucleotide-binding oligomerization domain protein 8

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

8H2, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... NLRP10(338322)

General description

NACHT, LRR and PYD domains-containing protein 10 (NLRP10) is also called Nucleotide-binding oligomerization domain protein 8. NLRP10 has anti-inflammatory activity and plays a role in immune response against infections from bacteria including C.albicans by contributing to proinflammatory cytokine release. NLRP10 is widely expressed with the highest levels found in heart, brain and skeletal muscle, as well as liver, colon, dermis and epidermis.

Immunogen

Recombinant protein corresponding to human NLRP10.

Application

Anti-NLRP10 Antibody, clone 8H2 is a highly specific rat monoclonal antibody, that targets NLRP10 & has been tested in western blotting, Flow Cytometry & ICC.
Immunocytochemistry Analysis: A representative lot from an independent laboratory detected NLRP10 in Flag-tagged NRLP10 transfected HeLa cells (Lautz, K., et al. (2012). Cell Microbiol. 14(10:1568-1583.).

Western Blotting Analysis: A representative lot from an independent laboratory detected NLRP10 primary epidermal fibroblasts transfected with a non-targeting control siRNA, and demonstrated a loss of signal in primary epidermal fibroblasts transfected with NLRP10-specific RNA (Lautz, K., et al. (2012). Cell Microbiol. 14(10:1568-1583.).

Flow Cytometry Analysis: 4 µg from a representative lot detected NLRP10 in 1X10E6 HeLa and Jurkat cells.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 1 mg/mL of this antibody detected NLRP10 in 10 µg of A431 cell lysate.

Target description

~72 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
A431 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Seok-Joo Lee et al.
Innate immunity, 23(7), 569-577 (2017-08-03)
This study investigated the pathogenesis of periodontitis and the role of nucleotide-binding oligomerization domain-like receptor protein 10 (NLRP10). The human oral epithelial cell line HOK-16B was infected with two periodontal pathogens, Tannerella forsythia and Fusobacterium nucleatum, at various MOIs. RT-PCR

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