MAB363
Anti-NMDAR1 Antibody, clone 54.1
clone 54.1, Chemicon®, from mouse
Synonym(s):
N-methyl-D-aspartate receptor channel, subunit zeta-1, N-methyl-D-aspartate receptor subunit NR1, NMDA receptor 1, glutamate [NMDA] receptor subunit zeta 1, glutamate receptor, ionotropic, N-methyl D-aspartate 1
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
54.1, monoclonal
species reactivity
rat, monkey, human, Xenopus
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
radioimmunoassay: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
frog ... Grin1(780048)
human ... GRIN1(2902)
rat ... Grin1(24408)
rhesus monkey ... Grin1(574380)
Related Categories
General description
Glutamate is a widely utilized excitatory neurotransmitter in mammalian brain. The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by kainate (KA) and a-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are referred to as non-NMDA receptors. Two distinct genes encode the NMDA receptor subunits, NR1 and NR2. The NR1 subunit is expressed in abundance in virtually all regions of the brain.
Specificity
Recognizes an epitope between amino acids 660-811 of the NMDAR1 receptor. Shows no cross-reactivity with NMDAR2, NMDAR3, NMDAR4 or NMDAR5.
Immunogen
Epitope: Extracellular loop between transmembrane regions III & IV.
Recombinant fusion protein containing GST and NMDAR1 aa. 660-811.
Application
Anti-NMDAR1 Antibody, clone 54.1 is an antibody against NMDAR1 for use in ELISA, IC, IH, IH(P), RIA.
Immunocytochemistry:
A previous lot of this antibody was used in IC.
RIA:
A previous lot of this antibody was used in RIA.
ELISA:
A previous lot of this antibody was used in ELISA.
Immunohistochemistry: 1:500-2000, on 2-4% paraformaldehyde fixed tissue: typically free-floating, frozen or vibratome sections 24-48 hours at 4°C. Paraffin embedded formalin fixed sections have also been used at similar dilutions and incubation times. Care should be taken not to over fix the tissues. The clone 54.1 has been used for both light and electron microscopy.
Western blot:
1:500 with 50 migrograms/lane of material. Recognizes a 103-116kDa band.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used in IC.
RIA:
A previous lot of this antibody was used in RIA.
ELISA:
A previous lot of this antibody was used in ELISA.
Immunohistochemistry: 1:500-2000, on 2-4% paraformaldehyde fixed tissue: typically free-floating, frozen or vibratome sections 24-48 hours at 4°C. Paraffin embedded formalin fixed sections have also been used at similar dilutions and incubation times. Care should be taken not to over fix the tissues. The clone 54.1 has been used for both light and electron microscopy.
Western blot:
1:500 with 50 migrograms/lane of material. Recognizes a 103-116kDa band.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurotransmitters & Receptors
Quality
Evaluated by immunohistochemistry on rat brain and spinal cord tissue.
Immunohistochemistry(paraffin) Analysis:
NMDAR1 representative staining pattern/morphology on rat spinal cord at 1:200 dilution. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Frozen section. IHC -Frozen Staining Examples: Rat Spinal Cord
Immunohistochemistry(paraffin) Analysis:
NMDAR1 representative staining pattern/morphology on rat spinal cord at 1:200 dilution. Immunoreactivity is seen as integral membrane and nerve fiber staining of neurons. Frozen section. IHC -Frozen Staining Examples: Rat Spinal Cord
Target description
103-116 kDa
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal IgG2a liquid in buffer PBS, no preservative.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Rat brain and spinal cord tissue
Rat brain and spinal cord tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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