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Key Documents

ABN200

Sigma-Aldrich

Anti-C-X-C chemokine receptor type 5 (CXCR5) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

C-X-C chemokine receptor type 5, CXC-R5, CXCR-5, Burkitt lymphoma receptor 1, Monocyte-derived receptor 15, MDR-15, CD185

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CXCR5(643)

General description

C-X-C chemokine receptor type 5 (CXCR5; Burkitt lymphoma receptor 1; Monocyte-derived receptor 15) is a G-protein coupled cytokine receptor that binds selectively to the CXCL13 (B lymphocyte chemoattractant) ligand, and mediates the development and function of the immune system. It is expressed in mature B cells, and facilitates the migration of B cells into follicles of the splenic white pulp, and enables deposition of antigen on follicular dendritic cells. It is also involved in the development of lymph nodes and Peyer’s patches. Previous studies have reported that the expression of CXCR5 increases in rheumatoid arthritis.

Specificity

This antibody recognizes the cytoplasmic domain of C-X-C chemokine receptor type 5 (CXCR5).

Immunogen

Epitope: Cytoplasmic domain
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human C-X-C chemokine receptor type 5 (CXCR5).

Application

Anti-C-X-C chemokine receptor type 5 (CXCR5) Antibody is an antibody against C-X-C chemokine receptor type 5 (CXCR5) for use in Western Blotting, IHC(P).
Immunohistochemistry Analysis: A 1:1,000 dilution from representative lot detected C-X-C chemokine receptor type 5 (CXCR5) in mouse frontal cortex neurons and mouse spleen lymphocytes.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Western Blot in SH-SY5Y cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected C-X-C chemokine receptor type 5 (CXCR5) in 10 µg of SH-SY5Y cell lysate.

Target description

~51 / 38 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing with molecular weights ~42 and ~37 kDa The calculated molecular weight of this protein is 42 kDa, but can be observed at ~51 kDa due to high glycosylation.

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
SH-SY5Y cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Hanyun Zhang et al.
Cancer research, 83(9), 1410-1425 (2023-03-01)
Beyond tertiary lymphoid structures, a significant number of immune-rich areas without germinal center-like structures are observed in non-small cell lung cancer. Here, we integrated transcriptomic data and digital pathology images to study the prognostic implications, spatial locations, and constitution of

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