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Key Documents

8.51026

Sigma-Aldrich

5-Carboxy-tetramethylrhodamine

Novabiochem®

Synonym(s):

5-Carboxy-tetramethylrhodamine, 5-TAMRA

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About This Item

Empirical Formula (Hill Notation):
C25H22N2O5
CAS Number:
Molecular Weight:
430.45
MDL number:
UNSPSC Code:
12352005
NACRES:
NA.22

Quality Level

product line

Novabiochem®

Assay

≥96.0% (HPLC)
≥97% (TLC)

form

powder

reaction suitability

reagent type: fluorescent-labelling reagent

manufacturer/tradename

Novabiochem®

application(s)

peptide synthesis

storage temp.

2-8°C

InChI

1S/C25H22N2O5/c1-26(2)15-6-9-18-21(12-15)32-22-13-16(27(3)4)7-10-19(22)23(18)17-8-5-14(24(28)29)11-20(17)25(30)31/h5-13H,1-4H3,(H-,28,29,30,31)

InChI key

YMZMTOFQCVHHFB-UHFFFAOYSA-N

General description

This fluorescent dye (λem555 nm, λex580 nm) is supplied as a single isomer, which ensures production of labeled products of defined chemical structure, as well as greatly simplifying purification and characterization.

Associated Protocols and Technical Articles
Chromogenic Labeling of Synthetic Peptides

Application

5-Carboxy-tetramethylrhodamine Novabiochem®. CAS 91809-66-4, molar mass 430.45 g/mol.

Linkage

Replaces: 01-63-0114

Analysis Note

Color (visual): dark violett to brown
Appearance of substance (visual): powder
Identity (IR): passes test
Purity (TLC (EtOAc/MeOH/H2O/DIPEA 60 : 30 : 10 : 3)): ≥ 97 %
Assay (HPLC, area%): ≥ 96.0 % (a/a)

To see the solvent systems used for TLC of Novabiochem® products please click here.

Legal Information

Novabiochem is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Chromogenic and fluorogenic derivatives are invaluable tools for biochemistry, having numerous applications in enzymology, protein chemistry, immunology and histochemistry.

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