17-10264
ChIPAb+ JHDM1B Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
JMJD, Lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B, JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein, F-box and leucine-rich repeat protein 10, Protein containing CXXC domain 2, Ju
About This Item
Recommended Products
biological source
rabbit
Quality Level
clone
polyclonal
purified by
affinity chromatography
species reactivity
horse, mouse, pig, human
species reactivity (predicted by homology)
porcine (based on 100% sequence homology), equine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), rat (based on 100% sequence homology), rabbit (based on 100% sequence homology)
manufacturer/tradename
ChIPAb+
Upstate®
technique(s)
ChIP: suitable
dot blot: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
1 of 4
This Item | 17-10263 | 17-648 | 17-685 |
---|---|---|---|
horse, mouse, pig, human | human | human, mouse | human, plant, vertebrates |
polyclonal | polyclonal | polyclonal | CMA312, monoclonal |
human ... KDM2B(84678) | - | human ... H3F3B(3021) | - |
ChIP: suitable, western blot: suitable, dot blot: suitable | ChIP: suitable, western blot: suitable | ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable | ChIP: suitable, western blot: suitable |
General description
The ChIPAb+JHDM1B set includes the JHDM1B antibody, a Normal Rabbit IgG, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The JHDM1B and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of JHDM1B -associated chromatin.
Immunogen
Application
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Normal Rabbit IgG or Anti-JHDM1B and the Magna ChIP A Kit (Cat. # 17-610). Successful immunoprecipitation of JHDM1B associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin as a positive locus, and β-actin promoter primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Western Blot Analysis:
Representative lot data.
HeLa nuclear extract was probed with Anti-JHDM1B (1 ug/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates JHDM1B (~165 kDa). (Figure 3). An uncharacterized band appears at ~109 kDa in some lysates.
Immunocytochemistry Analysis:
Representative lot data.
Confocal fluorescent analysis of NIH/3T3 and HeLa cells using Anti-JHDM1B (Red). Actin filaments have been labeled with Alexa Fluor 488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus. (Figure 4).
Epigenetics & Nuclear Function
Histones
Packaging
Quality
Representative lot data.
Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µL of either Normal Rabbit IgG or Anti-JHDM1B and the Magna ChIP® A Kit (Cat. # 17-610). Successful immunoprecipitation of JHDM1B associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin (Figure 1).
Please refer to the EZ-Magna ChIP A (Cat. # 17-408) or EZ-ChIP (Cat. # 17-371) protocol for experimental details.
Target description
Physical form
Anti-JHDM1B (Rabbit Polyclonal). One vial containing 50 µL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) and 150 mM NaCl and 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C.
Concentration: 0.7 mg/mL
Normal Rabbit IgG. One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
ChIP Primers, human β-globin. One vial containing 75 μL of each primer (5 μM) specific for the human β-globin promoter. Store at -20°C.
FOR: AGG ACA GGT ACG GCT GTC ATC
REV: TTT ATG CCC AGC CCT GGC TC
Storage and Stability
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Analysis Note
Includes normal rabbit IgG and primers specific for human β-globin.
Other Notes
Legal Information
Disclaimer
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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