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ISO2

Sigma-Aldrich

Mouse Monoclonal Antibody Isotyping Reagents

sufficient for 1000 tests (clones) (by ELISA), sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Synonym(s):

Mouse Antibody Isotyping Kit

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About This Item

MDL number:
MFCD08705607
UNSPSC Code:
12352203
NACRES:
NA.32

usage

sufficient for 1000 tests (clones) (by ELISA)
 sufficient for 40 tests (clones) (by immunodiffusion, ODD)

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

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General description

The Mouse Monoclonal Antibody Isotyping Reagents enable qualitative isotype determination of mouse monoclonal antibosies derived from hybridoma supernatant, ascites fluid or purified forms.

Specificity

ISO-2 contains 0.2 mL vials of subclass specific antibodies to all four mouse IgG subclasses, IgA, and IgM. Directions are included for using these reagents in ELISA and Ouchterlony assays to determine the subclass of monoclonal antibodies in ascites, culture supernatants, or purified preparations. Additional reagents may be required, such as conjugates for ELISA assays or agarose and buffer components for Ouchterlony assays.

Application

Mouse Monoclonal Antibody Isotyping Reagents has been used in monoclonal antibody isotype determination using enzyme-linked immunosorbent assay (ELISA). It may be used in Ouchterlony immunodiffusion assays.
Determination of the subclass of a monoclonal antibody is helpful for characterization of the antibody, for choosing detection reagents, and for deciding on a purification scheme.

Features and Benefits

  • May be used in a variety of assay formats
  • Suitable for all antibody forms
  • Determines all mouse IgG subclasses, IgA, and IgM

Kit Components Only

Product No.
Description
  • goat antisera to mouse IgA .2 mL
  • goat antisera to mouse IgG3 .2 mL
  • goat antisera to mouse IgG1 .2 mL
  • goat antisera to mouse IgG2a .2 mL
  • goat antisera to mouse IgG2b .2 mL
  • goat antisera to mouse IgM .2 mL

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

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Certificates of Analysis (COA)

Lot/Batch Number
0000218263
0000184924
088M4812V
037M4888V
046M4752V

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Zhang Ying et al.
Hybridoma (2005), 28(5), 349-354 (2009-10-28)
Amyloid beta-protein (Abeta) has been causally implicated in the neurodegenerative processes that accompany Alzheimer's disease. Soluble oligomers of the Abeta(1-42) fragment are thought to be significantly more neurotoxic than higher molecular weight aggregates. We report the isolation and characterization of
Masanori Onda et al.
Journal of immunology (Baltimore, Md. : 1950), 177(12), 8822-8834 (2006-12-05)
Recombinant immunotoxins composed of an Ab Fv fragment joined to a truncated portion of Pseudomonas exotoxin A (termed PE38) have been evaluated in clinical trials for the treatment of various human cancers. Immunotoxin therapy is very effective in hairy cell
Olga V Fedorova et al.
Journal of hypertension, 26(12), 2414-2425 (2008-11-15)
Levels of marinobufagenin (MBG), an endogenous bufadienolide Na/K-ATPase (NKA) inhibitor, increase in preeclampsia and in NaCl-sensitive hypertension. We tested a 3E9 monoclonal anti-MBG antibody (mAb) for the ability to lower blood pressure (BP) in NaCl-sensitive hypertension and to reverse the
Manu Kurian Mathew et al.
The Journal of veterinary medical science, 81(12), 1753-1762 (2019-10-28)
Equine influenza is a leading cause for respiratory illness in equines. Major control measures involve vaccination which requires continuous harmonization owing to antigenic drift. The present study focused on assessing the protective efficacy of an inactivated recombinant equine influenza virus
Preparation and Characterization of a Monoclonal Antibody with High Affinity for Soluble A β Oligomers
Ying,Z et al.
Hybridoma, 28(5) (2009)
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Protocols

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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