194434
ABTS™ Chromophore
Synonym(s):
ABTS™ Chromophore
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About This Item
Empirical Formula (Hill Notation):
C18H18N4O6S4
Molecular Weight:
514.62
UNSPSC Code:
41116133
NACRES:
NA.41
form
solution
Quality Level
contains
≤0.1% sodium azide as preservative
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
clearvery pale yellow
shipped in
ambient
storage temp.
2-8°C
General description
Peroxidase substrate that is soluble, safe, convenient and has good overall performance in ELISA. ABTS™ Chromophore develops intense blue-green color that can be measured at 405 nm to 410 nm. May be used for both kinetic and endpoint reactions.
Single component peroxidase substrate which is soluble, safe, convenient and has good overall performance for ELISA. In the presence of horseradish peroxidase (HRP) and hydrogen peroxide, ABTS is oxidized to an intense blue-green, water-soluble product. This product is a radical cation having an absorption maximum at 416 nm. Typically, product formation is measured spectrophotometrically at 405 nm, allowing a quantitative analysis of HRP-based systems. The color formation as a function of time may be monitored, or the reaction may be stopped by the addition of acid; since the acid does not alter the absorbance at 405 nm, one may perform both kinetic and endpoint measurements at the same wavelength.
Warning
Toxicity: Irritant (B)
Physical form
Contains optimal concentrations of ABTS and H₂O₂ in a citrate buffer (pH 4.0) with proprietary stabilizers.
Reconstitution
ABTS™ solution should be stored at 4°C, but must be warmed to room temperature before use. The solution should be protected from exposure to direct sunlight, and should be discarded if it becomes bright yellow or turbid.
Other Notes
Rodgriquez-Lopez, J.N., et al. 2000. Biochemistry39, 13201.
Legal Information
ABTS is a trademark of Boehringer Mannheim Corp.
ABTS is a trademark of Roche
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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