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1.00045

Millipore

MUP Selective Supplement

For the preparation of TOS-MUP agar, For the enumeration of Bifidobacteria

Synonym(s):

Selective supplement MUP, MUP supplement

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About This Item

UNSPSC Code:
41106212
NACRES:
NA.74
Pricing and availability is not currently available.

Agency

ISO 29981

Quality Level

200

form

lyophilized powder

technique(s)

microbiological culture: suitable

application(s)

food and beverages
microbiology

storage temp.

2-8°C

suitability

Bifidobacterium spp.

Related Categories

General description

MUP Selective Supplement contains lithium mupirocin in lyophilized form. Lithium mupirocin selectively inhibits the growth of typical lactic acid bacteria from dairy products, such as Lactobacilli, Lactococci, Streptococci and Leuconostocs, while Bifidobacteria can grow uninhibited.

Application

MUP Selective Supplement is used as a supplement for the preparation of TOS-MUP agar. It is suitable for the enumeration of Bifidobacteria in Dairy products (probiotics, yoghurts, yoghurt like products, and dried milk products such as Bifidobacteria containing infant milk formulae).

Linkage

Replaces: 1.00045.0017

Analysis Note

Appearance (clearness): clear
Testing as supplement in TOS-Propionat Agar (1.00043.0500).
Recovery on test medium
- Bifidobacterium animalis subsp. lactis ATCC 27536:
≥ 70 %
- Bifidobacterium breve ATCC 15700: ≥ 70 %
- Bifidobacterium longum ATCC 15707: ≥ 70 %
Growth
- Lactobacillus casei ATCC 393 (WDCM 00100):
no growth
- Lactobacillus delbrueckii subsp. bulgaricus ATCC 11842 (WDCM 00102): no growth
- Streptococcus salivarius subsp. thermophilus DSM 20259: no growth
Incubation: 72 hrs. at 37°C, anaerobic.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Certificates of Analysis (COA)

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Questions

1–2 of 2 Questions  

  1. What is the quantity of mupirocin per vial please? Does it require pre-dissolution in DMSO before use?

    1 answer

    1. The typical composition (per vial) is: Lithium mupirocin 25 mg.
      And the final concentration per liter is: Lithium mupirocin 50 mg (conforms with ISO 29981/IDF 220: 2010).

      The preparation is as follows:
      Add 25 ml of sterile, deionized water to the lyophilizate in the original vial and dissolve by carefully swirling. Add the solved supplement to the base culture medium that has been cooled to 48°C ± 1°C.

      Helpful?

  2. Hi! I have TOS agar in the glass tube about 18 ml. How do I put MUP supplement into 18 ml tube as the supplement is pallet and it's hard to weigh out?So If you could give me solution to make this supplement in solution, that would be great!

    1 answer

    1. The preparation instructions for the supplement state that 25 mL of sterile purified water should be added to the vial to dissolve. For usage, 5 mL of supplement solution is added to 95 mL of the agar base and then dispensed to tubes or plates. The 18 mL volume of agar base would require the addition of 947 µL of supplement solution.

      Helpful?

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