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07-274

Sigma-Aldrich

Anti-dimethyl-Histone H3 (Lys36) Antibody

serum, Upstate®

Synonym(s):

H3K36me2, Histone H3 (di methyl K36), H3 histone family, member T, histone 3, H3, histone cluster 3, H3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, Drosophila, Schizosaccharomyces pombe, Saccharomyces cerevisiae, vertebrates, human, chicken

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
dot blot: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

dimethylation (Lys36)

Gene Information

human ... H3C1(8350)
mouse ... H3C1(360198)

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

A broad species cross-reactivity is expected.
Recognizes methylated Histone H3 (Lys36), Mr 17 kDa as determined by Immunoblotting. Does not cross react with peptides containing dimethyl-histone H4 Lys20, nor Histone H3 Lys4, Lys9, or Lys27.

Immunogen

Epitope: Lys36
KLH-conjugated, synthetic peptide corresponding to amino acids 30-38 (CPATGGV(dimethyl-K)KP) of Histone H3 with a N-terminal C added for conjugation. The immunizing sequence is conserved from Tetrahymena to human.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-dimethyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect dimethyl-Histone H3 (Lys36) also known as H3K36me2, Histone H3 (di methyl K36).

Quality

Routinely evaluated by western blot on in acid-extracted proteins from sodium butyrate treated cells.

Western Blot Analysis:
A 1:1000 dilution of this lot detected methylated Histone H3 in acid-extracted proteins from untreated HeLa cells (Catalog # 13-112). The antibody did not detect recombinant Xenopus laevis histone H3 purified from E. coli (Catalog # 14-411).

Target description

17 kDa

Physical form

Rabbit polyclonal IgG immunodepleted antiserum in buffer containing 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC. Serum was passed over immunoaffinity column containing the non-modified peptide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Sodium butyrate treated, acid extracted HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression
Strahl, B. D., et al
Molecular and cellular biology, 22, 1298-1306 (2002)
Succinate inhibition of alpha-ketoglutarate-dependent enzymes in a yeast model of paraganglioma.
Smith, EH; Janknecht, R; Maher, LJ
Human Molecular Genetics null
Ni Li et al.
The Journal of clinical investigation, 127(4), 1284-1302 (2017-03-21)
Loss of phosphatase and tensin homolog (PTEN) and activation of the PI3K/AKT signaling pathway are hallmarks of prostate cancer (PCa). However, these alterations alone are insufficient for cells to acquire metastatic traits. Here, we have shown that the histone dimethyl
Hai-Ning Du et al.
The Journal of biological chemistry, 285(15), 11704-11713 (2010-02-09)
Set2-mediated H3 Lys(36) methylation is a histone modification that has been demonstrated to function in transcriptional elongation by recruiting the Rpd3S histone deacetylase complex to repress intragenic cryptic transcription. Recently, we identified a trans-histone pathway in which the interaction between
Rice jmjC domain-containing gene JMJ706 encodes H3K9 demethylase required for floral organ development.
Sun, Qianwen and Zhou, Dao-Xiu
Proceedings of the National Academy of Sciences of the USA, 105, 13679-13684 (2008)

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