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Key Documents

07-013

Sigma-Aldrich

Anti-MeCP2 Antibody

Upstate®, from rabbit

Synonym(s):

MeCP-2 protein, mental retardation, X-linked 16, mental retardation, X-linked 79, methyl CpG binding protein 2, methyl CpG binding protein 2 (Rett syndrome)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

rat, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

mouse ... Mecp2(17257)
rat ... Mecp2(29386)

General description

NA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the histone deacetylase protein families.

Specificity

Predicted to cross-react with human based on the conservation of the immunogenic sequence.
Recognizes MeCP2, Mr 75 kDa. A protein at ~75 kDa was also observed in HeLa nuclear extract. This protein was competed by pre-incubation of the antibody with the immunogenic peptide, suggesting that some HeLa strains contain MeCP2 or a related protein, contrary to a previous report.

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 465-478 of mouse MeCP2 (C-PRPNREEPVDSRTP) with an N-terminal cysteine added for conjugation purposes.

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-MeCP2 Antibody (rabbit polyclonal antibody) validated in WB to detect MeCP2 also known as MeCP-2 protein, mental retardation X-linked 16, mental retardation X-linked 79, methyl CpG binding protein 2.

Quality

Evaluated by Western Blot on Mouse Brain lysates.

Target description

~75 kDa observed

Physical form

Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse Brain lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marie Anaïs Labouesse et al.
Epigenetics, 10(12), 1143-1155 (2015-11-18)
Maternal infection during pregnancy increases the risk of neurodevelopmental disorders in the offspring. In addition to its influence on other neuronal systems, this early-life environmental adversity has been shown to negatively affect cortical γ-aminobutyric acid (GABA) functions in adult life
A WW domain binding region in methyl-CpG-binding protein MeCP2: impact on Rett syndrome.
Jan P Buschdorf, Wolf H Stratling
Journal of Molecular Medicine null
An Obligate Role for Membrane-associated Neutral Sphingomyelinase Activity in Orienting Chemotactic Migration of Human Neutrophils.
Sitrin RG, Sassanella TM, Petty HR
American Journal of Respiratory Cell and Molecular Biology null
Yuki Yagasaki et al.
PloS one, 13(5), e0198268-e0198268 (2018-05-31)
Loss or gain of copy number of the gene encoding the transcription factor methyl-CpG-binding protein 2 (MeCP2) leads to neurodevelopmental disorders (Rett and MeCP2 duplication syndrome), indicating that precisely regulated MeCP2 expression during development is critical for mental health. Consistent
SKI promotes Smad3 linker phosphorylations associated with the tumor-promoting trait of TGFbeta.
Lin, Q; Chen, D; Timchenko, NA; Medrano, EE
Cell Cycle null

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