Y1003
2x YT medium
Liquid microbial growth medium
Synonym(s):
2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium
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About This Item
Recommended Products
grade
for molecular biology
Quality Level
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
pH
6.5-7.5
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
General description
2xYT ( 2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.
Application
2x YT medium has been used for non-selective cultivation of M13 bacteriophage, E. coli strains for cloning , DNA plasmid production, and production of recombinant proteins. It is also suitable for selective cultivation when appropriate antibiotics are added.
Features and Benefits
2xYT liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package size
- Standard formulation
Components
16 g/L Tryptone
10 g/L Yeast Extract
5.0 g/L NaCl
10 g/L Yeast Extract
5.0 g/L NaCl
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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