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SRP3229

Sigma-Aldrich

PDGF-BB from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

Glioma-derived growth factor (GDGF), Osteosarcoma-derived Growth Factor (ODGF)

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

1.0-2.0 ng/mL ED50

mol wt

24.4 kDa

packaging

pkg of 10 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

off-white to yellow

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... PDGFB(18591)

General description

PDGFs (platelet-derived growth factors) are disulfide-linked dimers consisting of two 12.0-13.5kDa polypeptide chains, designated PDGF-A and PDGF-B chains. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-α and PDGFR-β. PDGFR-α is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-β interacts with only PDGF-BB and PDGF-AB. Recombinant murine PDGF-BB is a 24.4kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Application

PDGF-BB from mouse has been used as a chemotactic factor on vascular smooth muscle cell.

Biochem/physiol Actions

The three naturally occurring PDGFs (platelet-derived growth factors); PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet α-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Heterozygous mutation in PDGFB is associated with primary familial brain calcification. It is also associated with angiogenesis for osteogenesis. PDGFB induces migration of endothelial progenitor cells and mesenchymal stem cells.

Sequence

MSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT

Physical form

Lyophilized from 10 mM Sodium Citrate, pH 4.0.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Arterial smooth muscle cells express platelet-derived growth factor (PDGF) A chain mRNA, secrete a PDGF-like mitogen, and bind exogenous PDGF in a phenotype- and growth state-dependent manner.
Sjolund M, et al.
The Journal of Cell Biology, 106, 403-413 (1988)
PDGF-BB secreted by preosteoclasts induces angiogenesis during coupling with osteogenesis.
Xie H, et al.
Nature Medicine, 20, 1270-1278 (2014)
Mechanism of platelet-derived growth factor (PDGF) AA, AB, and BB binding to alpha and beta PDGF receptor.
Fretto LJ, et al.
The Journal of Biological Chemistry, 268, 3625-3631 (1993)
Roles of PDGF in animal development.
Hoch RV and Soriano P
Development, 130, 4769-4784 (2003)
Decorin inhibition of PDGF-stimulated vascular smooth muscle cell function: potential mechanism for inhibition of intimal hyperplasia after balloon angioplasty.
Nili N, et al.
The American Journal of Pathology, 163, 869-878 (2003)

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