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SRP3212

Sigma-Aldrich

IL-5 from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

BCDFII, EDF, TRF

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

mouse

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL ED50

mol wt

26.2 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... IL5(16191)

General description

IL-5 (interleukin 5) is a Th2 (T helper 2) cytokine that is predominantly produced by T lymphocytes, and to a lower extent by mast cells, type 2 innate lymphoid (ILC2) cells, and eosinophils.
Recombinant murine IL-5 is a 26.2kDa disulfide-linked homodimeric protein containing two 113 amino acid chains.

Biochem/physiol Actions

IL-5 (interleukin 5) was initially identified as a stimulant of antibody production by activated B cells. It has recently been shown to be a key factor in eosinophil differentiation and proliferation. It is also implicated in the pathogenesis of asthma, atopy, hypereosinophilic syndromes, and parasitic infections. In distal lung, IL-5 recruits eosinophils and modulates inflammatory or immune cells in the microenvironment, thus, promoting lung metastasis in cancer.

Sequence

MEIPMSTVVK ETLTQLSAHR ALLTSNETMR LPVPTHKNHQ LCIGEIFQGL DILKNQTVRG GTVEMLFQNL SLIKKYIDRQ KEKCGEERRR TRQFLDYLQE FLGVMSTEWA MEG

Physical form

Lyophilized from 10 mM Sodium Phosphate, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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