SAB4200723

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal

clone WH-3, purified from hybridoma cell culture

• Synonym(s): Anti-TPH, Anti-TpOH
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: WH-3, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~55 kDa
• species reactivity: mouse, human, rabbit, sheep, rat
• concentration: ~1.0 mg/mL
• technique(s): immunoblotting: 2-4 μg/mL using rabbit pineal gland lysate; immunofluorescence: suitable; immunohistochemistry: suitable
• isotype: IgG3
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the WH-3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with recombinant rabbit Tryptophan Hydroxylase. Tryptophan Hydroxylase (TPH), also known as TpOH, TrpH or L-tryptophan tetrahydropteridine: oxygen oxidoreductase (EC 1.14.16.4) is a member of biopterin-dependent aromatic amino acid hydroxylases family, together with tyrosine hydroxylase and phenylalanine hydroxylase. This gene spanning 29kbp with 11 exons is mapped to human chromosome 11p15.3-p14. This 444 amino acid protein is expressed in the gut, spleen, thymus and also in the pineal gland and the pituitary.

Immunogen

recombinant rabbit Tryptophan Hydroxylase.

Application

Anti-Tryptophan Hydroxylase antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunohistochemistry
• immunofluorescence

Biochem/physiol Actions

Tryptophan Hydroxylase (TPH) is a rate-limiting tetramer enzyme in the synthetic pathway of neurotransmitter serotonin from L-tryptophan and is considered a key factor for the maintenance of normal serotonin transmission in the central nervous system. The tryptophan hydroxylase 1 gene (TPH1) catalyzes the synthesis of 5-hydroxytryptophan. Mutation in the TPH1 gene leads to schizophrenia, irritable bowel syndrome (IBS) in women, suicidal behavior and depressive disorders.The encoded protein plays a vital role in regulation of cardiovascular function.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable