SAB4200119
Monoclonal Anti-FLAG-Peroxidase antibody produced in rat
2-4 mg/mL, clone 6F7, purified immunoglobulin
Synonym(s):
Anti-ddddk, Anti-dykddddk
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.32
clone:
6F7, monoclonal
application:
WB
species reactivity:
all
technique(s):
western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein
citations:
9
Pricing and availability is not currently available.
Recommended Products
biological source
rat
Quality Level
conjugate
peroxidase conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
6F7, monoclonal
form
buffered aqueous solution
species reactivity
all
concentration
2-4 mg/mL
technique(s)
western blot: 1:1,000-1:2,000 using extracts of transfected cells expressing C-terminal FLAG-tagged fusion protein
isotype
IgG1
immunogen sequence
DYKDDDDK
shipped in
dry ice
storage temp.
−20°C
General description
Monoclonal Anti-FLAG-Peroxidase is a purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP). The hybridoma 6F7 was produced by the fusion of mouse myeloma cells and splenocytes from rat immunized with the FLAG peptide.
The product recognizes N-terminal, C-terminal, and internal FLAG-tagged fusion proteins. It is especially recommended for identifying C-terminal FLAG-tagged fusion proteins.
Immunogen
purified immunoglobulin fraction of monoclonal Anti-FLAG (rat IgG1 isotype) isolated from culture supernatant of the 6F7 hybridoma cells grown in a bioreactor, conjugated to horseradish peroxidase (HRP).
Application
Learn more product details in our FLAG® applications portal.
Monoclonal Anti-FLAG-Peroxidase, recognizes N-terminal, C-terminal and internal FLAG-tagged fusion proteins. The product is especially recommended for identifying C-terminal FLAG-tagged fusion proteins. The product can be used for immunoblotting.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.01% merthiolate.
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Methods in molecular biology (Clifton, N.J.), 2690, 193-204 (2023-07-14)
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