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S9754

Sigma-Aldrich

Sulfatase from abalone entrails

Type VIII, lyophilized powder, 20-40 units/mg solid

Synonym(s):

Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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About This Item

CAS Number:
9016-17-5
Enzyme Commission number:
3.1.6.1 (BRENDA, IUBMB)
EC Number:
232-772-1
MDL number:
MFCD00132399
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

abalone entrails

Quality Level

200

type

Type VIII

form

lyophilized powder

specific activity

20-40 units/mg solid

mol wt

~43 kDa by gel filtration

foreign activity

β-Glucuronidase ≤10.0 Sigma units/mg solid

storage temp.

−20°C

Related Categories

Application

Sulfatase from abalone entrails has been used in a study to determine that human p-selectin glycoprotein ligand-1 interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. Sulfatase from abalone entrails has also been used in a study to investigate HPLC of sulfate and glutathione conjugates from hamster embryo fibroblasts.
Sulfatase from abalone entrails has been used to digest the conjugated forms into free forms for the analysis of tissue green tea polyphenols (GTPs). It has also been used for the detection of free, glucuronidated or total form of GTPs.

Biochem/physiol Actions

Sulfatases hydrolyze the sulfate esters of complex macromolecules, like glycosaminoglycans and sulfatides. It helps to modulate the sulfation states that determine the action of several physiological molecules.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sulfatases: structure, mechanism, biological activity, inhibition, and synthetic utility
Hanson SR, et al.
Angewandte Chemie (International ed. in English), 43(43), 5736-5763 (2004)
Green Tea Polyphenols and Metabolites in Prostatectomy Tissue: Implications for Cancer Prevention
Wang P, et al.
Cancer Prevention Research (Philadelphia, Pa.), 3(8), 985-993 (2010)
Quercetin increased bioavailability and decreased methylation of green tea polyphenols in vitro and in vivo
Wang P, et al.
Food, 3(6), 635-642 (2012)
Takako Hirata et al.
The Journal of biological chemistry, 279(50), 51775-51782 (2004-10-07)
P-selectin glycoprotein ligand-1 (PSGL-1), a sialomucin expressed on leukocytes, is a major ligand for P-selectin and mediates leukocyte rolling on the endothelium. Here we show that human PSGL-1 interacts with CCL27 (CTACK/ILC/ESkine), a skin-associated chemokine that attracts skin-homing T lymphocytes.
Amanda Cano et al.
Nanomedicine : nanotechnology, biology, and medicine, 14(4), 1073-1085 (2018-02-20)
Temporal lobe epilepsy is the most common type of pharmacoresistant epilepsy in adults. Epigallocatechin-3-gallate has aroused much interest because of its multiple therapeutic effects, but its instability compromises the potential effectiveness. PEGylated-PLGA nanoparticles of Epigallocatechin-3-gallate were designed to protect the
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Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

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