HT 1376

87032402, human bladder, Epithelial

• UNSPSC Code: 41106514

Properties

• Product Name: HT 1376, 87032402
• biological source: human bladder
• growth mode: Adherent
• karyotype: 2n = 46, hypertetrapoid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human Caucasian bladder carcinoma

Cell Line Description

Derived from a 58 year old caucasian female with a grade 3 transitional carcinoma of the bladder who had not received chemo or radiation therapy. The cells grow in soft agar and are tumorigenic in mice and hamsters. The patient donor had not received chemotherapy or radiation treatment.

Application

Tumourigenicity studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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