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14405C

SAFC

EX-CELL® 405 Serum-Free Medium for Insect Cells

with L-glutamine, liquid, sterile-filtered, suitable for insect cell culture

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About This Item

MDL number:
MFCD01634638
UNSPSC Code:
12352207
NACRES:
NA.75

description

for research or for further manufacturing use

sterility

sterile-filtered

form

liquid

quality

Drug Master File available

technique(s)

cell culture | insect: suitable

components

L-glutamine: yes: 4.717 g/L
NaHCO3: 0.33 g/L
glucose: 9.434 g/L
HEPES: no

shipped in

ambient

storage temp.

2-8°C

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Application

Baculoviruses are powerful expression systems for production of recombinant proteins. The most well known and highly investigated baculovirus vector expression system (BEVS) uses Spodoptera frugiperda cells (especially Sf9) as the host cell of choice for infection with recombinant Autographa californica nuclear polyhedrosis virus for heterologous protein expression. Recent investigations have shown that other cell lines may be better expression substrates than spodopteran cells. The BTI-TN-5B1-4 clone of Trichoplusia ni, commonly referred to as High Five™, is reported to be a superior host substrate for the expression of selected recombinant proteins using BEVS. This cell line portrays strong adherent characteristics, but can be adapted to suspension growth.

EX-CELL™ 405 is a serum-free medium for optimal growth and viability of BTI-TN-5B1-4 (Tn5, High Five) cells. In addition, EX-CELL 405 supports recombinant protein production in High Five cells to higher levels than reported for Sf9 cells. EX-CELL 405 is a complete medium. No protein supplements need to be added to this medium prior to use.

Legal Information

Available in USA, Canada, and Europe. Please check with your local supplier regarding availability and restrictions in other countries.
EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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M S Donaldson et al.
Biotechnology progress, 14(4), 573-579 (1998-08-08)
The BTI-Tn5B1-4 insect cell line, commercially available as the High Five cell line (Invitrogen), supports higher levels of recombinant protein production compared to existing insect cell lines. Proprietary serum-free media such as ExCell 405 (JRH Biosciences), Express Five (Life Technologies)
Gulzhakhan Sadvakassova et al.
BMC research notes, 2, 215-215 (2009-10-27)
C21, the C-terminal peptide of thrombospondin-4, has growth promoting activity and was discovered as one of several erythropoietin-dependent endothelial proteins. C21 stimulates red cell formation in anemic mice and is a growth factor for CD34+ and CD36+ hematopoietic cells, skin
R A Taticek et al.
Biotechnology progress, 17(4), 676-684 (2001-08-04)
Culture conditions required for obtaining maximum recombinant protein concentrations from two cell lines, Spodoptera frugiperda (IPLBeta-Sf21-AE) and Trichoplusia ni (Tn 5Beta-1-4), were determined in this work. Conditions studied include mode of culture (suspended vs attached), agitation rates, inoculum sizes, cell

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