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208694

Sigma-Aldrich

Calmodulin, Bovine Brain, High Purity

Calmodulin is a ubiquitous Ca2+-binding protein that serves as a physiological effector of a wide range of biological processes. Purified from bovine brain.

Synonym(s):

Calmodulin, Bovine Brain, High Purity, CaM, Ca 2+/Calmodulin, CaM, Ca2+/Calmodulin

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.77

biological source

bovine brain

Quality Level

Assay

≥95% (SDS-PAGE)

form

lyophilized

specific activity

≥250,000 units/mg protein

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

technique(s)

cell based assay: suitable

solubility

aqueous buffer: soluble
water: soluble

shipped in

wet ice

storage temp.

−20°C

Gene Information

bovine ... Calm(32659)

General description

Research area: Cell Signaling

Calmodulin (CaM) ranks among the most highly conserved protein sequences, with only histone proteins H4 and H3, actin B, and ubiquitin showing greater evolutionary conservation. CaM is a primarily helical protein with N- and C-terminal domains, each containing two paired Ca2+-binding EF-hand motifs. These motifs, with pentagonal-bipyramidal geometry, feature a conserved 12-amino acid sequence (positions 1–12) within a canonical helix-loop-helix structure.

Application

Calmodulin, Bovine Brain, High Purity has been used to study melatonin-calmodulin interaction and to activate Calcium–calmodulin (CaM)-dependent protein kinase IIa (CaMKII)a through in vitro incubation.

Biochem/physiol Actions

Calmodulin (CaM) is a ubiquitous Ca2+-binding protein distributed in eukaryotic cells that serves as a major physiological effector for a wide range of cellular responses. The Ca2+-calmodulin complex is involved in the maintenance of intracellular Ca2+ homeostasis, as well as in the regulation of the intracellular concentration of other second messengers, such as cyclic AMP and inositol trisphosphate. Mutations in the CaM sequence can result in diverse and potentially life-threatening pathologies such as ventricular tachycardia, congenital arrhythmia, and long QT syndrome. These mutations have the capacity to modify CaM′s ability to bind to Ca2+ and its target proteins and peptides, thereby disrupting their subsequent activity. Ca2+/CaM complex and CaM-binding domains (CaMBDs) interactions influence memory formation, muscle contraction, cellular metabolism, and cytoskeletal rearrangements.

Packaging

Yes

Warning

Toxicity: Standard Handling (A)

Unit Definition

One unit is defined as the amount of calmodulin that gives rise to 50% of the maximal enzyme activation of a standard level of activator-deficient calcineurin.

Physical form

Lyophilized from 30 mM HEPES, 1 mM CaCl₂, 0.1 mM DTT, pH 7.4.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C or -70°C). Aqueous stock solutions are stable for up to 2 months at -20°C or for up to 4 months at -70°C.
Please refer to the vial label for lot-specific reconstitution volume.

Other Notes

This product is not to be used for animal treatment, in vivo research or in any other contact procedure with livestock.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Olivia Appelmans et al.
Analytical biochemistry, 630, 114313-114313 (2021-07-25)
Radiometric assays have widely been used for measuring protein kinase activity for decades. In addition, several non-radiometric kinase assay formats have been developed over the years, including luciferase-based and fluorescence-based assays. However, radiometric assays are still considered as the "gold
Jesús Argueta et al.
International journal of molecular sciences, 23(5) (2022-03-11)
Melatonin (MEL) is a pleiotropic indolamine that reaches multiple intracellular targets. Among these, MEL binds to calmodulin (CaM) with high affinity. In presence of Ca2+, CaM binds to CaM-dependent kinase II (CaMKII). The Ca2+-CaM/CaMKII pathway regulates a myriad of brain
Corey Andrews et al.
International journal of molecular sciences, 22(1) (2021-01-06)
Calmodulin (CaM) is an important intracellular protein that binds Ca2+ and functions as a critical second messenger involved in numerous biological activities through extensive interactions with proteins and peptides. CaM's ability to adapt to binding targets with different structures is

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