07-274

Anti-dimethyl-Histone H3 (Lys36) Antibody

serum, Upstate^®

• Synonym(s): H3K36me2, Histone H3 (di methyl K36), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse, Drosophila, Schizosaccharomyces pombe, Saccharomyces cerevisiae, vertebrates, human, chicken
• manufacturer/tradename: Upstate^®
• technique(s): ChIP: suitable; dot blot: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_003493
• UniProt accession no.: Q16695
• shipped in: wet ice
• target post-translational modification: dimethylation (Lys36)
• Gene Information: human ... H3C1(8350) ; mouse ... H3C1(360198)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Specificity

A broad species cross-reactivity is expected.

Recognizes methylated Histone H3 (Lys36), Mr 17 kDa as determined by Immunoblotting. Does not cross react with peptides containing dimethyl-histone H4 Lys20, nor Histone H3 Lys4, Lys9, or Lys27.

Immunogen

Epitope: Lys36

KLH-conjugated, synthetic peptide corresponding to amino acids 30-38 (CPATGGV(dimethyl-K)KP) of Histone H3 with a N-terminal C added for conjugation. The immunizing sequence is conserved from Tetrahymena to human.

Application

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-dimethyl-Histone H3 (Lys36) Antibody (Rabbit Polyclonal Antibody) validated in WB, DB to detect dimethyl-Histone H3 (Lys36) also known as H3K36me2, Histone H3 (di methyl K36).

Quality

Routinely evaluated by western blot on in acid-extracted proteins from sodium butyrate treated cells.

Western Blot Analysis:
A 1:1000 dilution of this lot detected methylated Histone H3 in acid-extracted proteins from untreated HeLa cells (Catalog # 13-112). The antibody did not detect recombinant Xenopus laevis histone H3 purified from E. coli (Catalog # 14-411).

Target description

17 kDa

Physical form

Rabbit polyclonal IgG immunodepleted antiserum in buffer containing 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC. Serum was passed over immunoaffinity column containing the non-modified peptide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Analysis Note

Control
Sodium butyrate treated, acid extracted HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1