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198285

Sigma-Aldrich

Nigrosin

certified by the Biological Stain Commission

Synonym(s):

Acid black 2, Nigrosin water soluble

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About This Item

CAS Number:
Colour Index Number:
50420
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47
Pricing and availability is not currently available.

grade

certified by the Biological Stain Commission

Quality Level

form

solid

solubility

H2O: 0.1%
H2O: soluble

λmax

570 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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Suitability

Certified for use in Dorner′s spore stain. Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.
Used as a negative stain in place of India ink for spirochetes, bacteria, protozoa and fungi.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number

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To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm. Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was
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The Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections. The major problem of the technique is that a few hours after the

Questions

1–8 of 8 Questions  
  1. What is the extinction coefficient for Nigrosin?

    1 answer
    1. regarding the extinction coefficient for product 198285,t due to the uncertain composition of the dye, providing the extinction coefficient is not feasible.

      Helpful?

  2. During the certification testing for Product 198285, Nigrosin, what organism is used for testing?

    1 answer
    1. The exact organism can vary, but typically a 2-4 day old culture of a rapid spore former such as Bacillus cereus is utilized.

      Helpful?

  3. Is Product 198285, Nigrosin, soluble in ethanol?

    1 answer
    1. Solubility is stated to be only 0.0 to 0.4% in ethanol.

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  4. What is the molecular weight for Product 198285, Nigrosin?

    1 answer
    1. No formula weight or empirical formula is available. The dye is innately a mixture of dyes.

      Helpful?

  5. Has Product 198285, Nigrosin, been used to test for sperm viability?

    1 answer
    1. Yes. 10% Nigrosin Water Soluble is often used with 5% aqueous Eosin Y to determine live versus dead sperm. A procedure is given in Kjeldsberg, CR and Knight JA; Body Fluids, Second Edition (ASCP Press, Chicago 1986), p 168.

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  6. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  7. What is the solubility of Product 198285, Nigrosin, in water?

    1 answer
    1. The maximum solubility in water can vary from 5 to 10%, depending upon the lot number.

      Helpful?

  8. What is the purity of Product 198285, Nigrosin, Water Soluble?

    1 answer
    1. No dye purity is determined because the dye is a complex mixture of dyes with an unknown constitution.

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