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SAB3700541

Sigma-Aldrich

Anti-Rat IgG (Fc specific)-Peroxidase antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym(s):

HRP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

lyophilized powder

species reactivity

rat

technique(s)

immunohistochemistry: suitable
indirect ELISA: suitable
western blot: suitable

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by disulfide bond. It is a glycoprotein and a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG is further divided as- IgG1, IgG2a, IgG2b and IgG2c.

Specificity

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose.  Assay by immunoelectrophoresis resulted in a single precipitin arc against Anti-Peroxidase, Anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab′)2.

Immunogen

Rat IgG F(c) fragment

Biochem/physiol Actions

Immunoglobulin G (IgG) mainly helps in immune defense. It participates in hypersensitivity type II and type III. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Physical properties

Antibody format: IgG

Physical form

Supplied in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution

Reconstitute with 1.0 mL deionized water (or equivalent).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Specific IgG for cat allergens in patients with allergic conjunctivitis.
Miyama A, et al.
International Ophthalmology, 35(4), 575-586 (2015)
Antibody structure, instability, and formulation.
Wang W
Journal of Pharmaceutical Sciences, 96(1), 1-26 (2007)

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