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RAB0507

Sigma-Aldrich

Human VEGF-A ELISA Kit

for serum, plasma, cell culture supernatants and urine

Synonym(s):

VEGF-A, Vascular endothelial growth factor A 

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.32

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type plasma
sample type cell culture supernatant(s)
sample type serum
sample type urine

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... VEGFA(7422)

General description

The Human VEGF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human VEGF in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human VEGF-A

Application

For research use only. Not for use in diagnostic procedures.
Human VEGF (vascular endothelial growth factor) ELISA Kit has been used to measure VEGFA levels in human umbilical vascular endothelial cells (HUVECs) incubated with plasma from pre-eclamptic and healthy pregnant women.
Human VEGF-A ELISA Kit has been used to qualify:
  • VEGF in human liver hepatoma cell line PLC-PRF-5,
  • residual cytokines in decellularized meningeal scaffolds (DMS)
  • human periodontal ligament (hPDL) fibroblasts

Biochem/physiol Actions

VEGF (vascular endothelial growth factor) is a potent angiogenic cytokine. It stimulates proliferation and migration of endothelial cells. VEGF induces microvascular permeability by enhancing function of vesicular-vacuolar organelle (VVO). VEGF plays a prominent role in both normal and pathological angiogenesis. Substantial evidence suggests that VEGF is involved in the induction of tumor metastasis and intraocular neovascular syndromes. Increased expression of VEGF causes retinal angiogenesis, thus, inhibition of VEGF using soluble VEGF-receptor chimeric proteins can be considered as a potential therapy for ischemic retinal disease. Polymorphism in VEGF at C (-634) G is linked with diabetic retinopathy in type 2 diabetes. Altered expression of VEGF is also associated with rheumatoid arthritis, Graves′ disease, psoriasis, cardiovascular disease, and peripheral limb disease.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS
  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
  • RABSTOP3ELISA Stop Solution (Item I)SDS
  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
  • RABWASH420X Wash Buffer (Item B)SDS

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290

Precautionary Statements

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible corrosive hazardous materials

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Plasma from pre-eclamptic patients induces the expression of the anti-angiogenic miR-195-5p in endothelial cells.
Sandrim VC
Journal of Cellular and Molecular Medicine, 20(6), 1198-1200 (2016)
Yue Zhao et al.
Evidence-based complementary and alternative medicine : eCAM, 2020, 5204917-5204917 (2020-05-19)
To evaluate the efficacy of Abelmoschus manihot in treating type 2 diabetic nonproliferative retinopathy. It was a randomized controlled clinical trial. The recruited eighty subjects with type 2 diabetic nonproliferative retinopathy were randomly divided into treatment group and control group.
Exogenous hydrogen sulfide promotes hepatocellular carcinoma cell growth by activating the STAT3-COX-2 signaling pathway
Zhen Y, et al.
Oncology Letters, 15(5), 6562-6570 (2018)
Yaoyao Luo et al.
Acta pharmaceutica Sinica. B, 10(11), 2227-2245 (2020-12-12)
The clinical application of triptolide (TPL) in tumor therapy has been greatly limited by its toxicity and inefficient delivery. Herein, a localized and sustained-release thermo-sensitive hydrogel was developed for the intra-tumor administration of TPL. Based on the amphiphilic structure of
Daniel Chiumia et al.
International journal of molecular sciences, 21(2) (2020-01-19)
Vascular endothelial growth factor A (VEGFA) plays a critical angiogenic role in the endometrium of placentalia during preimplantation. The role of VEGFA and its receptors is not fully characterised in bovine reproduction. We analysed the mRNA expression of VEGFA isoforms
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