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P1867

Sigma-Aldrich

Plasmin from human plasma

Plasmin from human plasma
1 of 1 reviewers received a sample product or took part in a promotion

lyophilized powder, ≥2.0 units/mg protein

Synonym(s):

Fibrinolysin

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About This Item

CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
42030239
NACRES:
NA.54
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

specific activity

≥2.0 units/mg protein

impurities

HIV, Hepatitis B and Hepatitis C, tested negative

UniProt accession no.

application(s)

diagnostic assay manufacturing

storage temp.

−20°C

Gene Information

human ... PLG(5340)

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General description

Plasmin functions as a key enzyme of the fibrinolytic cascade, and is also important in inflammation processes. [1]

Application

A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. [2] It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin. [3]

Biochem/physiol Actions

It is inhibited by α 2-antiplasmin and its interaction with fibrin is blocked. [4] It digests the N-terminal region of the cytoplasmic protein αsynuclein preventing its uptake by surrounding cells. [5]
Plasmin exhibits preferential cleavage at the carboxyl side of lysine and arginine residues with higher selectivity than trypsin. Converts polymerized fibrin into soluble products.
pH Optimum: 8.5
pH 7.5: ~40% of maximal activity, pH 9.5: ~50% of maximal activity
Temperature Optimum: 37 °C with rapid inactivation at 56 °C

Packaging

Package size based on protein content

Unit Definition

One unit will produce one μmole of p-Nitroanilide from D-Val-Leu-Lys-p-Nitroanilide per minute at pH 7.5 at 37 °C.

Physical form

Lyophilized powder containing sodium phosphate and Trehalose.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Certificates of Analysis (COA)

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Customers Also Viewed

S Müllertz et al.
The Biochemical journal, 159(3), 545-553 (1976-12-01)
A complex between plasmin and an inhibitor was isolated by affinity chromatography from urokinase-activated human plasma. The complex did not react with antibodies against any of the known proteinase inhibitors in plasma. A rabbit antiserum against the complex was produced.
Tatiana Syrovets et al.
Journal of leukocyte biology, 92(3), 509-519 (2012-05-09)
The serine protease plasmin generated from its zymogen plasminogen is best known for its function as a key enzyme of the fibrinolytic cascade. However, beyond fibrinolysis, plasmin has a number of crucial functions in a variety of processes, including inflammation.
Efrat Shavit-Stein et al.
PloS one, 16(3), e0248431-e0248431 (2021-03-16)
Ischemic stroke is a common and debilitating disease with limited treatment options. Protease activated receptor 1 (PAR1) is a fundamental cell signaling mediator in the central nervous system (CNS). It can be activated by many proteases including thrombin and plasmin
Peter Stalmans et al.
Investigative ophthalmology & visual science, 54(10), 6620-6627 (2013-08-29)
To characterize the levels of active ocriplasmin over a period of ascending time points (range, 5 minutes to 7 days) from intravitreal injection of a 125-μg dose to sampling. During this 7-week controlled, open-label, phase 2 study, a single intravitreal
Dejun Yang et al.
Proceedings of the National Academy of Sciences of the United States of America, 118(33) (2021-08-13)
Idiopathic normal pressure hydrocephalus (iNPH) is a common neurological disorder that is characterized by enlarged cerebral ventricles, gait difficulty, incontinence, and dementia. iNPH usually develops after the sixth decade of life in previously asymptomatic individuals. We recently reported that loss-of-function

Protocols

Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride

Questions

1–9 of 9 Questions  
  1. Can you provide a method of production for Product P1867 - Plasmin?

    1 answer
    1. Product P1867 - Plasmin from human plasma is from an outside supplier and the manufacturing process is considered proprietary. The product is activated with Streptokinase and this enzyme is removed in the final steps of the purification. The product is >95% by SDS PAGE. The product is >3% protein (the exact value will be on the lot specific Certificate of Analysis). This material is lyophilized from 20 mM sodium phosphate (pH 7.4), containing 25 mM Trehalose. The lyophyllization buffer will contribute a significant amount of solids to the product. The product package size is based on protein content.

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  2. Is more information available on the solubility of product P1867 to aid with its application?

    1 answer
    1. The solubility of this product is not part of the QC testing; however, according to literature data, it should dissolve at least up to 1 mg/ml in an aqueous solvent. This preparation is recommended to go into water.

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  3. Is the P1867-150UG product in Lys-Plasmin form or Glu-Plasmin form?

    1 answer
    1. Both the Glu- and Lys- forms are assumed to be present in the product, as amino-terminal peptides are released from the native Glu-form to the new Lys-form by plasmin in vivo and during purification. No separation of the two forms is performed once purified plasminogen has been converted to plasmin.

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  4. What is Product P1867, Plasmin from human plasma, soluble in?

    1 answer
    1. It is soluble in water.

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  5. Does Product P1867, Plasmin from human plasma, have a recommended retest date?

    1 answer
    1. If a product has a recommended retest date this would be on the Certificate of Analysis found at our website.  If no recommended retest date is found on the Certificate of Analysis, then the guarantee of quality is one year from the time of delivery for unopened bottles stored at the recommended temperature.

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  6. Can Product P1867, Plasmin from human plasma, be stored?

    1 answer
    1. Sigma has not evaluated the solution stability of plasmin solutions.It is best to prepare solutions fresh.

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  7. What is the molecular weight of Product P1867, Plasmin from human plasma?

    1 answer
    1. The molecular weight of this dimeric protein was reported to be 75,400 Daltons. Reference is: The Plasma Proteins, Volume 1, 2nd ed., Putnam, F.W., ed., p. 66 (1975).

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  8. Is there a conversion to International Units (I.U.) for Product P1867, Plasmin from human plasma?

    1 answer
    1. Sigma has not evaluated the activity of this enzyme in I.U., nor do we have a conversion factor.

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  9. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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Reviews

1 of 1 reviewers received a sample product or took part in a promotion

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    • Review 1
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    1 out of 5 stars.

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