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L6254

Sigma-Aldrich

Leishman′s stain

Powder

Synonym(s):

Eosin-polychrome methylene blue

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About This Item

CAS Number:
12627-53-1
EC Number:
235-732-1
MDL number:
MFCD00131498
UNSPSC Code:
12171500
NACRES:
NA.47

product name

Leishman′s stain, used as histology stain

form

powder

technique(s)

microbe id | staining: suitable

color

dark green to very dark green

solubility

methanol: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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General description

Leishman stain is a Romanowsky-type dye, similar to Wright stain. Leishman stain is used in hematology and tropical medicine to differentiate and identify leukocytes, malarial parasites, and trypanosomes. It is also known as eosin-polychrome methylene blue.

Application

Leishman′s stain has been used for staining blood smears to differentiate blood corpuscles, malarial parasites, trypanosomers, etc. It may be used as an alternative to Giemsa.Leishman’s stain has been used for the identification of macrophage nuclei in cells. It has also been used in hematology study.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Note on a Simple and Rapid Method of Producing Romanowsky Staining in Malarial and other Blood Films.
W B Leishman
British medical journal, 2(2125), 757-758 (1901-09-21)
Cytochemistry and morphology of granulocytes of the caecilian Siphonops annulatus (Amphibia, Gymnophiona).
Gutierre RC
Comparative clinical pathology, 17, 221-221 (2008)
D K Mendiratta et al.
Indian journal of medical microbiology, 24(1), 49-51 (2006-03-01)
Rapid diagnosis is a prerequisite for institution of effective treatment and reducing the mortality and morbidity of falciparum malaria. This study was taken up to compare the efficacy of various rapid methods viz, acridine orange, Plasmodium falciparum histidine rich protein
Cloning and expression of feline colony stimulating factor receptor (CSF-1R) and analysis of the species specificity of stimulation by colony stimulating factor-1 (CSF-1) and interleukin-34 (IL-34).
Gow DJ
Cytokine, 61, 630-630 (2013)
Pradeep A Subramani et al.
Malaria journal, 19(1), 214-214 (2020-06-24)
Vivax malaria is associated with significant morbidity and economic loss, and constitutes the bulk of malaria cases in large parts of Asia and South America as well as recent case reports in Africa. The widespread prevalence of vivax is a

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