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I7385

Sigma-Aldrich

Monoclonal Anti-Human IgG4 antibody produced in mouse

clone HP-6025, ascites fluid

Synonym(s):

Monoclonal Anti-Human IgG4

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

secondary antibodies

clone

HP-6025, monoclonal

contains

15 mM sodium azide

technique(s)

immunofluorescence: suitable
indirect ELISA: 1:5,000

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

IgG4 (Immunoglobulin G4) is produced by T helper 2 cytokines. Unlike other immunoglobulin G is rare and constitutes to only 3-4 percent of the total IgG in the serum.

Specificity

Monoclonal Anti-Human IgG4 is specific for the IgG4 subclass and nonreactive with IgG1, IgG2 and IgG3 in an ELISA.

Application

Monoclonal Anti-HuIgG4 man antibody produced in mouse has been used in ELISA and immunofluorescence.
The IUIS/WHO2 study singled out this monoclonal antibody as one of the most widely applicable IgG4 specific monoclonal antibodies. Monoclonal Anti-Human IgG4 may be used for the identification of the IgG4 subclass by various immunoassays including: ELISA, Imprint Immunofixation (IIF), Immunofluorometric Assay (IFMA), Hemagglutination (HA), Hemagglutination Inhibition (HAI), Particle Counting Immunoassay (PACIA), and detection of cytoplasmic IgG.

Biochem/physiol Actions

IgG4 (Immunoglobulin G4) antibody has reduced affinity for C1q (first component of complement q) and hence its anti-inflammatory activity differing from that of the other IgG subclasses. IgG4 antibodies are known to actively interchange Fab arms with another molecule, resulting in recombined antibodies with two different binding specificities. This exchange is considered as an important biological mechanism that contributes to its anti-inflammatory activity. IgG4 antibodies are normally noninflammatory, but autoreactive IgG4 antibodies are known to be associated with Immunoglobulin G4 (IgG4)-related disease (IgG4-RD), characterized by infiltrating lymphoplasmacytic cells and elevated serum IgG4. Increased levels of IgG4 is observed in atopic dermatitis, parasitic diseases, pemphigus vulgaris and pemphigus foliaceus.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Charge and size selectivity of proteinuria in children with idiopathic nephrotic syndrome.
Taylor G M, et al.
Pediatric Nephrology (Berlin), 11(4), 404-410 (1997)
High serum IgG4 concentrations in patients with sclerosing pancreatitis.
Hamano H, et al.
The New England Journal of Medicine, 344(10), 732-738 (2001)
Preclinical characterization of 1-7F9, a novel human anti?KIR receptor therapeutic antibody that augments natural killer?mediated killing of tumor cells.
Romagne F, et al.
Blood, 114(13), 2667-2677 (2009)
Shi-Jun Li et al.
Clinical journal of the American Society of Nephrology : CJASN, 5(3), 439-444 (2010-01-22)
Long-term contact with mercury may induce membranous nephropathy (MN); however, the clinical pathologic features and pathogenesis of mercury-induced MN have not been investigated. The present study retrospectively evaluated 11 cases of mercury-induced MN to analyze its causes and its clinical
Anti-inflammatory activity of human IgG4 antibodies by dynamic Fab arm exchange.
Van Der Neut Kolfschoten M, et al.
Science, 317(5844), 1554-1557 (2007)

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