Skip to Content
Merck
All Photos(2)

Documents

C7479

Sigma-Aldrich

Anti-Calretinin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-CAB29, Anti-CAL2, Anti-CR

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 29 kDa

species reactivity

rat, human, chicken

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using formalin-fixed, paraffin-embedded rat cerebellum sections
microarray: suitable
western blot: 1:1,000 using recombinant human calretinin

UniProt accession no.

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CALB2(794)
rat ... Calb2(117059)

General description

Anti-Calretinin is developed in rabbit using a synthetic peptide corresponding to the C-terminal region of rat calretinin (amino acids 194-209) conjugated to keyhole limpet hemocyanin (KLH) as immunogen. Calretinin is an intracellular Ca2+-binding protein, with broad tissue distribution and is a member of the calmodulin superfamily. It is expressed in certain populations of neurons in the central and peripheral nervous systems. It is abundant in the cerebellum (granule cells), olfactory bulb and auditory pathways.

Immunogen

synthetic peptide corresponding to the C-terminal region of rat calretinin (amino acids 194-209) conjugated to KLH. This sequence is specific for calretinin and not found in other members of the EF-hand family such as calbindin-D-28K, calbindin-D-9K, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is identical in human and mouse calretinin and highly conserved in chicken calretinin.

Application

Anti-Calretinin antibody produced in rabbit has been used in immunohistochemistry and immunofluorescence.
Anti-Calretinin antibody produced in rabbit is suitable for the following applications:
  • immunohistochemistry
  • microarray
  • western blotting at a dilution of 1:1,000 using recombinant human calretinin

Biochem/physiol Actions

The physiological function of calretinin is not known, but it seems to be related to Ca2+ buffering and diffusion. Calretinin-deficient mice show impaired long-term potentiation (LTP) in the dentate gyrus, impaired motor coordination and Purkinje cell excitability. A role for both calretinin and calbindin-D-28K in synaptic plasticity and in neuroprotection from excitotoxic insults has been postulated. By acting as a buffer or sensor of intracellular free Ca2+, calretinin promotes calcium homeostasis. Furthermore, several truncated forms of calretinin have been found in certain tumor cells, but are absent from normal cells, suggesting that calretinin may play a role in tumorigenesis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Customers Also Viewed

Calretinin: from a ?simple? Ca2+ buffer to a multifunctional protein implicated in many biological processes
Schwaller B
Frontiers in Neuroanatomy, 8(3), 3-3 (2014)
Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin
Schiffmann SN, et al.
Proceedings of the National Academy of Sciences of the USA, 96(9), 5257-5262 (1999)
Inhibition of Glycogen Synthase Kinase 3 beta Blocks Mesomesenchymal Transition and Attenuates Streptococcus pneumonia-Mediated Pleural Injury in Mice
Boren J, et al.
The American Journal of Pathology, 187(11), 2461-2472 (2017)
Immunocytochemical detection of calretinin in the claustrum and endopiriform nucleus of the chinchilla
Szalak R, et al.
Polish Journal of Veterinary Sciences, 18(4), 857-863 (2015)
Calretinin: a gene for a novel calcium-binding protein expressed principally in neurons.
Rogers JH
The Journal of Cell Biology, 105(3), 1343-1353 (1987)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service