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63590

Sigma-Aldrich

May-Grünwald solution

Synonym(s):

Eosin – methylene blue solution, Eosin methylene blue according to May-Grünwald

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About This Item

MDL number:
MFCD00005038
UNSPSC Code:
12171500
PubChem Substance ID:
329759877
NACRES:
NA.25

technique(s)

microbe id | staining: suitable

Quality Level

100

impurities

methanol

density

0.80 g/mL at 20 °C

suitability

Chlamydia spp.
Histoplasma spp.
Plasmodium spp.
Trichomonas spp.
bacteria
protozoa
spirochetes

SMILES string

[K+].COC(=O)c1ccccc1C2=C3C=C(Br)C(=O)C(Br)=C3Oc4c(Br)c([O-])c(Br)cc24

InChI

1S/C21H10Br4O5.K/c1-29-21(28)9-5-3-2-4-8(9)14-10-6-12(22)17(26)15(24)19(10)30-20-11(14)7-13(23)18(27)16(20)25;/h2-7,26H,1H3;/q;+1/p-1

InChI key

NXBZXYJXNIFCRJ-UHFFFAOYSA-M

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Application

May-Grünwald solution (Eosin – methylene blue solution) is used with Giemsa stain for histological staining of blood cells.

Other Notes

Stain for the panoptic staining according to Pappenheim

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1

Target Organs

Eyes,Central nervous system

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

51.8 °F - closed cup

Flash Point(C)

11 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

EU REACH Annex XVII (Restriction List)

CAS No.

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM0740
BCCL8222
BCCM1501
BCCK2940
BCCK2934

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Involvement of intrinsic mitochondrial pathway in neosergeolide-induced apoptosis of human HL-60 leukemia cells: The role of mitochondrial permeability transition pore and DNA damage.
Cavalcanti BC, da Costa PM, et al.
L' Eurobiologiste, 50, 980-993 (2012)
P. Bock, ed.
Romeis Mikroskopische Technik null
V Hofman et al.
Cytopathology : official journal of the British Society for Clinical Cytology, 23(1), 30-38 (2011-01-08)
Recurrence rates after surgery for non-small cell lung cancer (NSCLC) range from 25 to 50% and 5-year survival is only 60-70%. Because no biomarkers are predictive of recurrence or the onset of metastasis, pathological TNM (pTNM) staging is currently the
Elsa Beraki et al.
CytoJournal, 9, 8-8 (2012-05-11)
Protocols for immunocytochemical staining (ICC) and in situ hybridization (ISH) of air-dried Diff-Quick or May-Grünwald Giemsa (MGG)-stained smears have been difficult to establish. An increasing need to be able to use prestained slides for ICC and ISH in specific cases
Farshad Naghshvar et al.
Cell biochemistry and function, 30(7), 569-573 (2012-04-27)
The protective effects of carnosine as a natural dipeptide were investigated in mouse bone marrow cells against genotoxicity induced by cyclophosphamide. Mice were injected with solutions of carnosine at three different doses (10, 50 and 100 mg kg(-1) bw) for
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