We have data from our QC labs here at Sigma on one of the Genistin products we offer, G0897. The solvent is 85% ethanol/15% water, and the lambda max is typically 262.0 or 262.5 nm. We measured the molar extinction coefficients at the lambda max for four different lots of G0897 back in the 1990s, and the average value was 39,800 M-1.Genistin is a derivative of Genistein. There is an entry for Genistein in the chemicals encyclopedia published by the Royal Society of Chemistry, with information for Genistin under that heading.According to the chemicals encyclopedia published by the Royal Society of Chemistry, 14th edition, entry #4391, uv max (85% ethanol) 262.5 nm (a 90.5)".The value of ""a 90.5"" is the same as an E0.1%, or the extinction coefficient for a 1 mg/mL (or 1 gram per Liter) solution.If one multiplies 90.5 (L/g) by the molecular weight of genistin (432.38 g/mole), you get 39,130 M-1. This is pretty close to the value of 39,800 from our data above."
73822
Genistin
analytical standard
Synonym(s):
4′,5,7-Trihydroxyisoflavone 7-glucoside, Genistein 7-glucoside, Genistein-7-O-β-D-glucopyranoside, Genistoside, NSC 5112
About This Item
Recommended Products
grade
analytical standard
Quality Level
Assay
≥98.0% (HPLC)
shelf life
limited shelf life, expiry date on the label
technique(s)
HPLC: suitable
gas chromatography (GC): suitable
application(s)
food and beverages
format
neat
storage temp.
2-8°C
SMILES string
OC[C@H]1O[C@@H](Oc2cc(O)c3C(=O)C(=COc3c2)c4ccc(O)cc4)[C@H](O)[C@@H](O)[C@@H]1O
InChI
1S/C21H20O10/c22-7-15-18(26)19(27)20(28)21(31-15)30-11-5-13(24)16-14(6-11)29-8-12(17(16)25)9-1-3-10(23)4-2-9/h1-6,8,15,18-24,26-28H,7H2/t15-,18-,19+,20-,21-/m1/s1
InChI key
ZCOLJUOHXJRHDI-CMWLGVBASA-N
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General description
Application
- Legumes by ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS) with electrospray ionization (ESI) source and multiple reaction monitoring (MRM) detection.[2]
It may be used as an analytical reference standard for the determination of genistin in:
- Soy-based foods and dietary supplements by LC coupled to absorbance detection and isotope-dilution mass spectrometry (IDMS), equipped with ESI source and selected ion monitoring (SIM) mode.[3]
- Soy products by colloidal gold-based indirect competitive immunochromatographic assay (ICA).[1]
- Rainbow trout muscle by QuEChERS (quick, easy, cheap, effective, rugged and safe) extraction and LC-ESI-MS/MS.[4]
- Soybean flour by matrix solid phase dispersion extraction (MSPD) and LC with ultraviolet-diode array detection (UV-DAD).[5]
- Soymilk samples by HPLC-UV operating under the gradient elution mode.[6]
Biochem/physiol Actions
Packaging
Other Notes
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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