HGAMMAG-303E

MILLIPLEX^® Human Immunoglobulin IgE Single Plex Magnetic Bead Kit - Isotyping Assay

Isotyping Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous analysis of multiple immunoglobulins (Ig) in human serum, plasma and cell culture samples.

• Synonym(s): Human IgE Bead Kit, Human IgE Detection Kit, Immunoglobulin IgE Detection
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• Quality Level: 200
• species reactivity: human
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 102%; (IgE); sensitivity: 0.4 ng/mL; standard curve range: 4.1-1,000 ng/mL; (IgE)
• technique(s): multiplexing: suitable
• compatibility: configured for Single Plex
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

Found only in mammals, Immunoglobulin E (IgE) plays an essential role in allergic reactions and is predominantly associated with type 1 hypersensitivity. IgE is the least abundant immunoglobulin isotype in normal individuals, present at only 0.05% of IgG serum concentrations. It is capable of triggering robust immune reactions, with up to 10 times the typical level detected in the serum of atopic patients, although increased levels occur only in approximately 50% of patients with allergic disorders. IgE binds with high affinity to FcεRI, found primarily on mast cells and basophils, priming the cells to recognize specific antigens such as dust mites, pet dander or pollen. Once primed and with repeated exposure to the same antigen, these cells release molecules such as histamines, prostaglandins, and leukotrienes, resulting in an “allergic reaction”.

Although the mechanisms of this immune response are well understood, continued research is needed to understand the varying degrees of allergic responses – from mild rhinitis to anaphylaxis. Consequently, the MILLIPLEX^® Human IgE Bead Single Plex Kit enables you to measure levels of IgE in only 50 μL of 1:50 diluted human serum or plasma, saving both time and precious sample.

Panel Type: Immune Response

Specificity

Cross-reactivity between these antibodies and those against other immunoglobulins is non-detectable or negligible.

Application

• Analyte: IgE
• Recommended Sample type: Serum, plasma, or tissue/cell lysate and culture supernatant samples
• Recommended Sample dilution: 1:50 diluted plasma or serum, or 50 μL cell culture supernatant
• Assay Run Time: One day
• Research Category: Inflammation & Immunology

Packaging

96 Well Plate

Components

MILLIPLEX MAP Anti-Human k and λ Light Chain, PE

MILLIPLEX MAP Human IgE Standard

MILLIPLEX MAP Human Serum Positive Control

MILLIPLEX MAP Assay Buffer

MILLIPLEX MAP Washer Buffer, 10X

Set of one 96-Well Filter Plate with 4 Sealers

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Legal Information

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H332,H311,H317,H318,H373,H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects