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SRP3156

Sigma-Aldrich

Sonic Hedgehog human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):

HHG-1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

0.8-1.0 μg/mL ED50

mol wt

20.0 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... SHH(6469)

General description

The sonic hedgehog protein is encoded by the SHH gene that is mapped to human chromosome 7q36. It is homologous to the Drosophila segment polarity gene hedgehog. The encoded protein is a 45kDa precursor protein that is auto-cleaved to form SSH-N (an amino-terminal 20kDa product) and SSH-C (carboxy-terminal product). SSH-N is found to be cell-associated, while SSH-C diffuses freely. Recombinant E. coli derived Human Sonic HedgeHog is a 20.0 kDa protein consisting of 176 amino acid residues, including an N-terminal Ile-Val-Ile sequence substituted for the naturally occurring chemically modified Cys residue.

Application

Sonic Hedgehog human has been used:
  • To study its role in liver cancer stem cell activity.
  • For neuronal differentiation.
  • For cholinergic-like neurons (ChLNs) differentiation.

The sonic hedgehog protein is used in neuronal induction medium as a supplement.

Biochem/physiol Actions

The SHH (sonic hedgehog) gene encodes a soluble signaling protein that functions in the patterning of cell differentiation in the neural tube and limb bud. It has also been implicated in several processes, such as developing nervous system, including midbrain and ventral forebrain neuronal differentiation and neuronal precursor proliferation. In adults, it regulates progenitor function and proliferation. Mutations in this gene have been associated with holoprosencephaly, a developmental defect of the forebrain and midface.

Sequence

IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Physical form

Lyophilized with no additives.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mutations in the human Sonic Hedgehog gene cause holoprosencephaly.
Roessler E
Nature Genetics, 14, 357-360 (1996)
Use of human induced pluripotent stem cell-derived neurons as a model for cerebral toxoplasmosis
Tanaka N, et al.
Microbes and Infection, 18(7-8), 496-504 (2016)
Use of human induced pluripotent stem cell-derived neurons as a model for Cerebral Toxoplasmosis.
Tanaka N
Microbes and Infection, 18, 496-504 (2016)
The SHH/Gli axis regulates CD 90-mediated liver cancer stem cell function by activating the IL 6/JAK 2 pathway
Zhang K, et al.
Journal of Cellular and Molecular Medicine, 22(7), 3679-3690 (2018)
Direct transdifferentiation of human Wharton?s Jelly mesenchymal stromal cells into Cholinergic-like neurons
Mendivil-Perez M, et al.
Journal of Neuroscience Methods (2018)

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